Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QLG17_RS20415 Genome accession   NZ_CP124819
Coordinates   4095014..4096447 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain C325     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4090014..4101447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLG17_RS20380 (QLG17_20380) elbB 4090706..4091359 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  QLG17_RS20385 (QLG17_20385) mtgA 4091356..4092084 (+) 729 WP_000047097.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  QLG17_RS20390 (QLG17_20390) yrbL 4092081..4092713 (-) 633 WP_000620410.1 PhoP regulatory network protein YrbL -
  QLG17_RS20395 (QLG17_20395) npr 4092926..4093198 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  QLG17_RS20400 (QLG17_20400) rapZ 4093195..4094049 (-) 855 WP_000243741.1 RNase adapter RapZ -
  QLG17_RS20405 (QLG17_20405) ptsN 4094095..4094586 (-) 492 WP_001400765.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QLG17_RS20410 (QLG17_20410) hpf 4094704..4094991 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  QLG17_RS20415 (QLG17_20415) rpoN/rpoN1 4095014..4096447 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  QLG17_RS20420 (QLG17_20420) lptB 4096495..4097220 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  QLG17_RS20425 (QLG17_20425) lptA 4097227..4097784 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  QLG17_RS20430 (QLG17_20430) lptC 4097753..4098328 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  QLG17_RS20435 (QLG17_20435) kdsC 4098325..4098891 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QLG17_RS20440 (QLG17_20440) kdsD 4098912..4099898 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  QLG17_RS20445 (QLG17_20445) yrbG 4099912..4100889 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=761437 QLG17_RS20415 WP_000809051.1 4095014..4096447(-) (rpoN/rpoN1) [Escherichia coli strain C325]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=761437 QLG17_RS20415 WP_000809051.1 4095014..4096447(-) (rpoN/rpoN1) [Escherichia coli strain C325]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTTCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACGCCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAAGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAGCCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465