Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OOK74_RS10290 Genome accession   NZ_CP113241
Coordinates   2239177..2240193 (-) Length   338 a.a.
NCBI ID   WP_278182741.1    Uniprot ID   -
Organism   Vibrio sp. gvc     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2234177..2245193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOK74_RS10265 aroK 2235063..2235587 (-) 525 WP_166014237.1 shikimate kinase AroK -
  OOK74_RS10270 pilQ 2235774..2237486 (-) 1713 WP_274722625.1 type IV pilus secretin PilQ family protein Machinery gene
  OOK74_RS10275 pilP 2237512..2238030 (-) 519 WP_278182739.1 pilus assembly protein PilP Machinery gene
  OOK74_RS10280 pilO 2238020..2238610 (-) 591 WP_278182740.1 type 4a pilus biogenesis protein PilO Machinery gene
  OOK74_RS10285 pilN 2238603..2239187 (-) 585 WP_274722622.1 PilN domain-containing protein Machinery gene
  OOK74_RS10290 pilM 2239177..2240193 (-) 1017 WP_278182741.1 type IV pilus assembly protein PilM Machinery gene
  OOK74_RS10295 - 2240345..2242822 (+) 2478 WP_278182742.1 penicillin-binding protein 1A -
  OOK74_RS10300 oxyR 2242875..2243780 (-) 906 WP_278182743.1 DNA-binding transcriptional regulator OxyR -
  OOK74_RS10305 - 2243919..2244650 (+) 732 WP_274722618.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37604.21 Da        Isoelectric Point: 6.4129

>NTDB_id=761116 OOK74_RS10290 WP_278182741.1 2239177..2240193(-) (pilM) [Vibrio sp. gvc]
MGKSLVTGIDIGHHSIKAVVLKPMGDTCALVGYEELLVTADIFTDNYTLDYQKIVKKLKQLKKGLPLFSRKVALAIPDNA
VISKSLPIDSDLEPREQEFAIYQAFSHQSPFPVEDLSFDFVEIANSTVARSSTTTFQVYATRTEVVDSRLHVSKKAGFEP
ILIDVQVHSLLKLWQHASRALRRADWMLIDIGYSQSTLCLDWPDKMPFYKDIPFGTQRLDVEPRSEFAVNPLALQDPIQR
FIDELVDKTARQIQLFTSVHGAQHLSGLWLSGGGALMAGLTEALQQRLGLPCEVLDPFSLFTIDVARRQRPVRDGAQFST
AAGLALRGLAWLEGEHAG

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=761116 OOK74_RS10290 WP_278182741.1 2239177..2240193(-) (pilM) [Vibrio sp. gvc]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCATCACAGCATTAAAGCTGTGGTGCTCAAACCTATGGGCGACAC
CTGCGCGCTGGTGGGGTATGAGGAGTTGCTCGTTACCGCGGATATTTTCACCGATAACTACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTCAAACAACTAAAAAAGGGGTTACCGCTATTTAGTCGTAAAGTCGCATTGGCAATTCCGGATAATGCG
GTGATCAGCAAATCATTACCCATCGATAGTGACCTTGAACCACGTGAGCAGGAGTTTGCTATCTATCAGGCTTTCTCCCA
TCAATCCCCTTTTCCGGTCGAAGATCTGAGTTTCGATTTTGTCGAGATTGCGAATAGTACTGTTGCGCGCAGTAGCACGA
CAACCTTCCAAGTTTATGCCACCAGAACTGAAGTGGTCGACAGTCGTTTACACGTCAGTAAAAAAGCGGGTTTTGAGCCG
ATCTTGATCGATGTACAGGTGCATAGCTTGTTGAAGTTATGGCAACACGCGAGTCGTGCTCTGCGGCGTGCAGATTGGAT
GTTGATTGATATTGGCTATAGCCAAAGCACCCTGTGCCTCGATTGGCCAGACAAAATGCCATTTTACAAAGATATTCCGT
TCGGTACTCAACGACTCGACGTCGAACCAAGGAGCGAATTCGCCGTTAACCCATTAGCGCTGCAAGATCCAATACAGCGC
TTTATTGATGAGTTGGTCGATAAAACGGCCCGTCAAATTCAGTTATTCACCTCGGTACATGGGGCGCAACATCTGAGTGG
TCTATGGCTTTCTGGTGGTGGCGCACTGATGGCGGGTTTGACTGAGGCCCTACAGCAGCGTTTAGGGCTACCCTGTGAGG
TATTGGATCCATTCTCACTGTTCACAATCGATGTAGCGAGGCGTCAGCGACCAGTGAGGGATGGGGCACAGTTTAGTACC
GCGGCAGGTTTGGCCTTGCGTGGTTTAGCGTGGCTGGAGGGTGAGCATGCTGGATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)

Type IV pilus assembly protein PilM; (PilM_2)

(239-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

76.627

100

0.766
  pilM Vibrio campbellii strain DS40M4

44.412

100

0.447