Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QKA38_RS12600 Genome accession   NZ_CP124669
Coordinates   2646475..2648202 (+) Length   575 a.a.
NCBI ID   WP_023091667.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2021CK-01494     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2641475..2653202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QKA38_RS12580 (QKA38_12580) - 2642187..2643116 (-) 930 WP_003160458.1 LysR family transcriptional regulator -
  QKA38_RS12585 (QKA38_12585) - 2643139..2644113 (-) 975 WP_003110096.1 quinone oxidoreductase -
  QKA38_RS12590 (QKA38_12590) - 2644339..2645091 (+) 753 WP_003138896.1 class I SAM-dependent methyltransferase -
  QKA38_RS12595 (QKA38_12595) - 2645682..2646485 (+) 804 WP_003097732.1 ABC transporter permease -
  QKA38_RS12600 (QKA38_12600) pilF 2646475..2648202 (+) 1728 WP_023091667.1 GspE/PulE family protein Machinery gene
  QKA38_RS12605 (QKA38_12605) - 2648207..2649394 (+) 1188 WP_003138899.1 type II secretion system F family protein -
  QKA38_RS12610 (QKA38_12610) gspG 2649448..2649882 (+) 435 WP_003090519.1 type II secretion system major pseudopilin GspG -
  QKA38_RS12615 (QKA38_12615) - 2649851..2650261 (+) 411 WP_023981094.1 type II secretion system protein -
  QKA38_RS12620 (QKA38_12620) - 2650261..2650686 (+) 426 WP_003110087.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QKA38_RS12625 (QKA38_12625) - 2650683..2651273 (+) 591 WP_004350096.1 type II secretion system protein -
  QKA38_RS12630 (QKA38_12630) - 2651263..2652342 (+) 1080 WP_003138906.1 type II secretion system protein GspK -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63642.71 Da        Isoelectric Point: 5.1633

>NTDB_id=761055 QKA38_RS12600 WP_023091667.1 2646475..2648202(+) (pilF) [Pseudomonas aeruginosa strain 2021CK-01494]
MSSEAVSPGPQVTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLCLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TEAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=761055 QKA38_RS12600 WP_023091667.1 2646475..2648202(+) (pilF) [Pseudomonas aeruginosa strain 2021CK-01494]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGCCTGGGAGGTATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGCACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTT
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGTGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACGACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAGTACCAGGTGCCCAACATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGTGCCTTCACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACTGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)

Type II/IV secretion system protein (T2SSE)

(194-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.696

98.435

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.801

98.609

0.383
  pilF Neisseria gonorrhoeae MS11

36.842

99.13

0.365