Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QJ960_RS12285 Genome accession   NZ_CP124664
Coordinates   2554800..2556527 (+) Length   575 a.a.
NCBI ID   WP_052159536.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2021CK-01256     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2549800..2561527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QJ960_RS12265 (QJ960_12265) - 2550852..2551604 (+) 753 WP_003113396.1 class I SAM-dependent methyltransferase -
  QJ960_RS12270 (QJ960_12270) - 2551628..2552419 (-) 792 WP_003110094.1 alpha/beta hydrolase -
  QJ960_RS12275 (QJ960_12275) - 2552597..2553559 (+) 963 WP_223822180.1 helix-turn-helix domain-containing protein -
  QJ960_RS12280 (QJ960_12280) - 2554007..2554810 (+) 804 WP_003097732.1 ABC transporter permease -
  QJ960_RS12285 (QJ960_12285) pilF 2554800..2556527 (+) 1728 WP_052159536.1 GspE/PulE family protein Machinery gene
  QJ960_RS12290 (QJ960_12290) - 2556532..2557719 (+) 1188 WP_034048048.1 type II secretion system F family protein -
  QJ960_RS12295 (QJ960_12295) gspG 2557773..2558207 (+) 435 WP_003110089.1 type II secretion system major pseudopilin GspG -
  QJ960_RS12300 (QJ960_12300) - 2558176..2558586 (+) 411 WP_003097723.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QJ960_RS12305 (QJ960_12305) - 2558586..2559011 (+) 426 WP_003116709.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QJ960_RS12310 (QJ960_12310) - 2559008..2559598 (+) 591 WP_003097720.1 type II secretion system protein -
  QJ960_RS12315 (QJ960_12315) - 2559588..2560667 (+) 1080 WP_003119955.1 type II secretion system protein GspK -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63723.82 Da        Isoelectric Point: 5.2438

>NTDB_id=760559 QJ960_RS12285 WP_052159536.1 2554800..2556527(+) (pilF) [Pseudomonas aeruginosa strain 2021CK-01256]
MSSEAVSPGPQVTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TEVATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=760559 QJ960_RS12285 WP_052159536.1 2554800..2556527(+) (pilF) [Pseudomonas aeruginosa strain 2021CK-01256]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGTCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTAGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAACAGTT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCAGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTC
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACGACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAATACCAGGTGCCCAACATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGCGCCTTCACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCGAGGTGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)

Type II/IV secretion system protein (T2SSE)

(194-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.696

98.435

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.421

99.13

0.381
  pilF Neisseria gonorrhoeae MS11

36.842

99.13

0.365