Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   OVY33_RS06530 Genome accession   NZ_CP113107
Coordinates   1328587..1329933 (-) Length   448 a.a.
NCBI ID   WP_000816499.1    Uniprot ID   Q9R3W9
Organism   Staphylococcus rostri strain PJFA-333     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 1308770..1329933 1328587..1329933 within 0


Gene organization within MGE regions


Location: 1308770..1329933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OVY33_RS06430 mecA 1308770..1310779 (-) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  OVY33_RS06435 mecR1 1310876..1312633 (+) 1758 WP_309306387.1 beta-lactam sensor/signal transducer MecR1 -
  OVY33_RS06440 mecI 1312633..1313004 (+) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -
  OVY33_RS06445 psm-mec 1313089..1313157 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  OVY33_RS06450 - 1313516..1314625 (+) 1110 WP_309306790.1 ROK family transcriptional regulator -
  OVY33_RS06455 cstB 1314739..1316073 (-) 1335 Protein_1235 persulfide dioxygenase-sulfurtransferase CstB -
  OVY33_RS06460 - 1316106..1317170 (-) 1065 WP_309306388.1 DsrE/DsrF/DrsH-like family protein -
  OVY33_RS06465 cstR 1317306..1317566 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  OVY33_RS06470 - 1317566..1318204 (+) 639 Protein_1238 sulfite exporter TauE/SafE family protein -
  OVY33_RS06475 - 1318478..1319095 (-) 618 WP_000562107.1 CadD family cadmium resistance transporter -
  OVY33_RS06480 - 1319176..1321590 (-) 2415 WP_000378485.1 cadmium-translocating P-type ATPase CadA -
  OVY33_RS06485 - 1321583..1321948 (-) 366 WP_000159127.1 metalloregulator ArsR/SmtB family transcription factor -
  OVY33_RS06490 - 1322186..1322563 (-) 378 WP_001041908.1 DUF6262 family protein -
  OVY33_RS06495 - 1322570..1324462 (-) 1893 WP_309306389.1 site-specific integrase -
  OVY33_RS06500 - 1324657..1324980 (-) 324 WP_000088091.1 JAB domain-containing protein -
  OVY33_RS06505 - 1324973..1325179 (-) 207 WP_001077283.1 hypothetical protein -
  OVY33_RS06510 - 1325181..1325702 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  OVY33_RS06515 - 1325721..1326032 (-) 312 WP_000833212.1 DUF960 family protein -
  OVY33_RS06520 - 1326117..1326467 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  OVY33_RS06525 ccrB 1326938..1328566 (-) 1629 WP_001186596.1 cassette chromosome recombinase CcrB Machinery gene
  OVY33_RS06530 ccrA 1328587..1329933 (-) 1347 WP_000816499.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 52138.19 Da        Isoelectric Point: 10.1760

>NTDB_id=760293 OVY33_RS06530 WP_000816499.1 1328587..1329933(-) (ccrA) [Staphylococcus rostri strain PJFA-333]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQNIQYISDKQSGRTDNRTGYQQVTERIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFMSLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYHYQ
NGTFIINNDESPTVKAVFNYYLQGYGYKKIAQYLEDDNKLITRKPYQVRNIIMNPNYCGRVINQYGQYNNMVPPIVSATK
YEHAQAIRNKKQLHCIPSENQLKQKIKCPCCDSTLTNMTIRKKHTLRYYICPKNMNESRFVCSFKGINAQKLEVQVLATC
QNFFQNQQLYSKINNAIHQRLKKQRVIEAKSTLTQEQLIDKLAKGMIDAESFRKQTHLMNQKHKTISSISDNQLQTSLQK
VIQKSFTLNMLHPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=760293 OVY33_RS06530 WP_000816499.1 1328587..1329933(-) (ccrA) [Staphylococcus rostri strain PJFA-333]
ATGAAACGAGCCATTGGTTATTTGCGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCAATAGAATT
ATTAGCTCCAAAGCACAATATTCAAAATATCCAATACATTAGTGATAAGCAATCAGGCAGAACAGATAATCGAACAGGCT
ATCAACAAGTCACCGAACGCATCCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTTCATCGCAAC
TTGAAAAATGCATTAAAACTCATGAAACTCTGTCAAAAATATCATGTTCATATTCTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTCAATATATTCATGAGTCTGGCTGAACTTGAATCCGATAATATTGGAGAAC
AAGTCAAAAATGGACTTAGAGAAAAGGCAAAACAAGGTAAACTCATAACGACCCATGCGCCTTTCGGTTATCACTATCAA
AATGGTACTTTCATCATTAATAATGATGAATCACCTACCGTCAAAGCTGTATTCAATTATTATCTTCAAGGATATGGCTA
CAAGAAGATTGCACAATATTTAGAAGACGATAATAAACTTATTACCCGCAAGCCTTATCAGGTACGAAATATAATTATGA
ACCCAAATTATTGTGGTCGTGTCATCAATCAATATGGTCAATATAACAATATGGTACCACCTATTGTTTCGGCAACGAAA
TATGAACATGCTCAAGCAATCCGTAATAAGAAGCAACTTCACTGTATACCTTCAGAGAATCAGCTGAAACAAAAGATCAA
ATGTCCTTGTTGTGACTCAACACTGACAAATATGACAATAAGAAAAAAACATACATTGCGATATTATATTTGTCCTAAAA
ATATGAATGAATCTCGCTTTGTCTGTTCATTCAAAGGAATAAATGCACAAAAATTAGAAGTTCAAGTCTTAGCTACATGT
CAGAACTTCTTTCAAAACCAACAGCTCTATTCAAAAATTAATAATGCAATTCATCAACGCCTCAAAAAACAAAGAGTGAT
AGAAGCTAAAAGTACGCTAACTCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAATCATTCAGAA
AACAGACTCATTTGATGAATCAAAAGCACAAAACCATATCCTCCATAAGTGATAATCAGTTACAAACATCACTACAAAAG
GTTATACAGAAAAGTTTCACGTTAAACATGCTGCATCCCTATATTGATGAAATTCGCATTACAAAAAATAAAGCCCTTGT
TGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(168-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9R3W9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

78.174

100

0.783
  ccrA Staphylococcus aureus N315

72.383

100

0.725