Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   OS085_RS12650 Genome accession   NZ_CP113044
Coordinates   2533653..2534150 (-) Length   165 a.a.
NCBI ID   WP_267836530.1    Uniprot ID   -
Organism   Staphylococcus aureus strain LeBlanc     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2528653..2539150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OS085_RS12625 gcvPB 2528815..2530287 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  OS085_RS12630 gcvPA 2530280..2531626 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  OS085_RS12635 gcvT 2531646..2532737 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  OS085_RS12640 - 2532896..2533420 (-) 525 WP_001015117.1 shikimate kinase -
  OS085_RS12645 - 2533410..2533556 (-) 147 WP_001789879.1 hypothetical protein -
  OS085_RS12650 comGF 2533653..2534150 (-) 498 WP_267836530.1 competence type IV pilus minor pilin ComGF Machinery gene
  OS085_RS12655 comGE 2534068..2534367 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  OS085_RS12660 comGD 2534354..2534800 (-) 447 WP_001791635.1 competence type IV pilus minor pilin ComGD Machinery gene
  OS085_RS12665 comGC 2534778..2535089 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  OS085_RS12670 comGB 2535103..2536173 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  OS085_RS12675 comGA 2536145..2537119 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  OS085_RS12680 - 2537171..2537794 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  OS085_RS12685 - 2537791..2538120 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  OS085_RS12690 - 2538120..2539106 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19180.92 Da        Isoelectric Point: 10.2192

>NTDB_id=759463 OS085_RS12650 WP_267836530.1 2533653..2534150(-) (comGF) [Staphylococcus aureus strain LeBlanc]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVLDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=759463 OS085_RS12650 WP_267836530.1 2533653..2534150(-) (comGF) [Staphylococcus aureus strain LeBlanc]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCTAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-52)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

99.394

100

0.994
  comGF Staphylococcus aureus N315

95.758

100

0.958