Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   P7I84_RS27355 Genome accession   NZ_CP124638
Coordinates   5887934..5888557 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 2021CK-01158     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5882934..5893557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I84_RS27335 (P7I84_27345) aroB 5883529..5884635 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  P7I84_RS27340 (P7I84_27350) aroK 5884685..5885203 (-) 519 WP_003095833.1 shikimate kinase AroK -
  P7I84_RS27345 (P7I84_27355) pilQ 5885215..5887359 (-) 2145 WP_003135767.1 type 4a pilus secretin PilQ Machinery gene
  P7I84_RS27350 (P7I84_27360) comL/pilP 5887413..5887937 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  P7I84_RS27355 (P7I84_27365) pilO 5887934..5888557 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  P7I84_RS27360 (P7I84_27370) pilN 5888554..5889150 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  P7I84_RS27365 (P7I84_27375) pilM 5889150..5890214 (-) 1065 WP_034074518.1 type IV pilus assembly protein PilM Machinery gene
  P7I84_RS27370 (P7I84_27380) - 5890399..5892870 (+) 2472 WP_034074516.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=759202 P7I84_RS27355 WP_003103268.1 5887934..5888557(-) (pilO) [Pseudomonas aeruginosa strain 2021CK-01158]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=759202 P7I84_RS27355 WP_003103268.1 5887934..5888557(-) (pilO) [Pseudomonas aeruginosa strain 2021CK-01158]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTCC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401