Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ORU25_RS06070 Genome accession   NZ_CP111145
Coordinates   1194096..1194641 (+) Length   181 a.a.
NCBI ID   WP_010907447.1    Uniprot ID   -
Organism   Pasteurella multocida strain PF9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1189096..1199641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORU25_RS06060 (ORU25_06060) - 1190327..1192693 (+) 2367 WP_010907445.1 tetratricopeptide repeat protein -
  ORU25_RS06065 (ORU25_06065) - 1192873..1194057 (+) 1185 WP_010907446.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ORU25_RS06070 (ORU25_06070) pilF/pilF2 1194096..1194641 (+) 546 WP_010907447.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ORU25_RS06075 (ORU25_06075) - 1194764..1195720 (+) 957 WP_005725132.1 helix-turn-helix domain-containing protein -
  ORU25_RS06080 (ORU25_06080) ispG 1195747..1196850 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ORU25_RS06085 (ORU25_06085) hisS 1196880..1198151 (+) 1272 WP_010907448.1 histidine--tRNA ligase -
  ORU25_RS06090 (ORU25_06090) - 1198170..1198781 (+) 612 WP_005725137.1 YfgM family protein -
  ORU25_RS06095 (ORU25_06095) - 1198986..1199423 (+) 438 WP_010907449.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20911.47 Da        Isoelectric Point: 7.8913

>NTDB_id=758161 ORU25_RS06070 WP_010907447.1 1194096..1194641(+) (pilF/pilF2) [Pasteurella multocida strain PF9]
MTRVFQVWIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDTVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=758161 ORU25_RS06070 WP_010907447.1 1194096..1194641(+) (pilF/pilF2) [Pasteurella multocida strain PF9]
ATGACGCGCGTTTTTCAAGTATGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCAGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACACTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50

97.238

0.486
  pilF Glaesserella parasuis strain SC1401

43.85

100

0.453