Detailed information NTG0756360

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: OPU65_RS08175
• Genome accession: NZ_CP110812
• Coordinates: 1570092..1573784 (-)
• Length: 1230 a.a.
• NCBI ID: WP_265627395.1
• Uniprot ID: -
• Organism: Bacillus paralicheniformis strain CamBx3
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1565092..1578784

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OPU65_RS08160 (OPU65_08160)	-	1565194..1565412 (+)	219	WP_016885923.1	spore germination protein	-
OPU65_RS08165 (OPU65_08165)	sbcC	1565457..1568849 (-)	3393	WP_265627393.1	exonuclease subunit SbcC	-
OPU65_RS08170 (OPU65_08170)	sbcD	1568846..1570027 (-)	1182	WP_265627394.1	exonuclease subunit SbcD	-
OPU65_RS08175 (OPU65_08175)	addA	1570092..1573784 (-)	3693	WP_265627395.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
OPU65_RS08180 (OPU65_08180)	addB	1573771..1577271 (-)	3501	WP_265627396.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
OPU65_RS08185 (OPU65_08185)	modB	1577381..1578037 (-)	657	WP_243939524.1	molybdate ABC transporter permease subunit	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141357.24 Da
• Isoelectric Point: 5.3354

>NTDB_id=756360 OPU65_RS08175 WP_265627395.1 1570092..1573784(-) (addA) [Bacillus paralicheniformis strain CamBx3]
MEISKPKGSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQERPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKQDPA
FFELVDRYTTDRHDLDLQHLVKRVYKFSRSHPDPEGWLHTFVDLYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPGGPAPRAENFLDDLAQIDRLIQQQNDFSALYELVPAVSFQRLKPCKGDAYDPRLVDEAADLRNSAKKQFEKLK
SDYFTRTPEQHLESLREMKPVIQTLVQLVLEYGKRFADAKKEKAIVDFSDLEHDCLAILSGKNQAGEAVPSEAAMFYRHQ
FDEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGATYPPNEATKTELLLIENQSGAQDEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKTYLSAADGDERLSEKLRRFDALLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDMNKSYLLDKELGFGTKFIHPKWRISYPMLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRSQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPLVIDEIREHPARFQVSWLSAADLQAE
DVDQAGEERHGRLAQIQMGLPIDGSFEYEQQVRERLTWSYPYNDAAKVRTKQSVSEMKRQKEYEDEYGDRSLIRPSKEAF
LFKRPSFMMAKGLTAAERGTAMHTVMQHIPLTRTPEINELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLLG
ARRVEREVPFNMTLSAKEVYPDLESADEPVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQ
LYARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide

• Length: 3693 bp

>NTDB_id=756360 OPU65_RS08175 WP_265627395.1 1570092..1573784(-) (addA) [Bacillus paralicheniformis strain CamBx3]
ATGGAGATTTCTAAACCGAAGGGCAGCACATGGACTGATGACCAATGGAAGGCGATCGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGCTCCGGAAAAACGGCCGTGCTGGTTGAGCGGATCATTCGGAAAATAACGGATCAGGAACGTC
CCGTAGATGTCGACAGGCTGCTTGTCGTCACCTTTACGAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCAGACAGCTTGCTTTATTAAATAAAGCGAGCATATC
AACATTGCACTCATTCTGTCTTCAAGTCATCCGCAAATACTATTATTTGATCGATGTTGATCCGGCTTTCCGGATCGCTG
ATCAGACGGAAGGCGAGCTGCTAGGCGATGAGGTGCTGGACGAGCTGTTTGAAGAAGAATACAAAAAGCAAGATCCGGCC
TTTTTTGAACTTGTGGACAGATATACGACAGACCGTCATGACCTCGATTTGCAGCATCTTGTCAAGCGGGTCTACAAGTT
TTCCCGTTCGCATCCCGATCCGGAAGGCTGGCTTCACACATTTGTTGATCTGTATGACGCGGCTTCAGATACGAAAGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAGGGGATGCGGCAAAAGCTGACCCGTGCG
CTTGATTTGACAAAGCAGCCCGGGGGGCCTGCTCCGCGCGCCGAAAACTTCCTCGATGATTTAGCACAGATTGACAGGCT
GATTCAGCAGCAGAATGATTTTTCGGCCCTGTACGAGCTTGTACCGGCCGTTTCGTTTCAAAGATTGAAGCCGTGCAAAG
GAGATGCGTACGATCCTCGATTGGTTGATGAGGCGGCAGACCTCCGCAACAGCGCGAAAAAGCAATTTGAAAAGCTGAAA
AGCGATTATTTTACGCGAACTCCCGAGCAGCACCTTGAGAGCCTGAGGGAAATGAAGCCGGTCATTCAGACGCTCGTCCA
GCTCGTGTTGGAGTATGGCAAGCGGTTTGCGGACGCGAAAAAGGAAAAAGCGATCGTCGATTTCTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGGGAAAAACCAAGCCGGCGAAGCCGTGCCGAGCGAAGCGGCGATGTTTTACCGCCATCAG
TTCGATGAAGTGCTTGTTGATGAATATCAGGATACGAACCTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCAGAACCGCTTTTGTTCC
TCTCGAAATATAAACGCTTTACAGACGATGGCTCGGGTTCCGGCCAGAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTTAAGCAGCTCATGGGAGAAAAGGTCGGGGAAGTCGAATATGACGAACA
AGCAGAATTGAAGCTTGGCGCTACTTACCCGCCGAACGAAGCGACAAAAACGGAGCTTCTGCTCATCGAAAACCAGAGCG
GCGCGCAGGATGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGGCAAATCCGCAGGCTG
ATAACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCGCGGAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGGTCGATGCCTTGGGCGCCGCAAATTATGGACGAGTTTAAACAGCAGGGGATCCCGGTATACGCGAACCTCTCAACGG
GTTATTTTGAAGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCTTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTAGGTCTTGACGAAAATGAGCTTTCCTTGATCAGAATCAAGGATAAAAAAGCGCC
GTTTTACGAAGCGATGAAAACATACCTTTCTGCAGCAGACGGCGATGAAAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
CCCTGCTCAAAAAGTGGAGAGCGTATGCGAAAAACCACTCTGTGGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGCGCTTTACGACAGGGCGAGATC
CTATGAAGCGACATCGTTCCGCGGTTTGTTCCGCTTTTTAAGATTTATTGAGCGAATGCAGGAGCGGGGTGATGATCTCG
GAACGGCAAGAGCTCTGAGTGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGACTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACATGAACAAGTCTTATCTGCTGGATAAAGAGCTCGG
TTTCGGAACGAAGTTTATTCATCCGAAGTGGCGGATCAGCTATCCGATGCTGCCGCTGATCGCGATGAAGAAAAAGCTCC
GCCGCGAGCTTTTATCAGAGGAGCTCCGCGTCTTGTATGTCGCCCTTACCCGGGCCAAGGAAAAGCTGTACCTGGTCGGA
ACGGCGAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGTCACAAGCGGCAGGGTCTGAGTGGCTGCTCCCGGATTATGA
ACGGTTTCAGGCGAAATCTTACCTGGATTTCATCGGGCCTGCTTTGATGAGACACAGAGATATGGATGAATCCGGCGCGC
CGCTAGTGATTGATGAGATCCGTGAACATCCGGCCCGCTTTCAGGTCAGCTGGCTGTCCGCCGCGGATCTTCAAGCAGAG
GATGTTGATCAGGCCGGTGAAGAAAGACACGGCCGGCTCGCCCAAATTCAAATGGGGCTTCCGATCGACGGTTCGTTTGA
GTATGAGCAACAAGTCAGAGAACGGCTGACATGGTCATATCCATACAATGACGCCGCCAAAGTCCGAACAAAACAGTCTG
TCTCAGAAATGAAAAGACAGAAGGAATATGAGGATGAATACGGCGACCGCTCGCTGATCCGCCCATCAAAGGAGGCATTC
CTGTTTAAGCGGCCGTCCTTTATGATGGCAAAAGGCCTGACTGCGGCCGAACGGGGAACGGCGATGCACACCGTCATGCA
GCATATTCCGCTGACCCGTACGCCTGAAATAAATGAGCTCAGCCGTCTCCTTGACAGACTTGTTGAGAAAGAGCTGTTAA
CAGAAGACCAGCGGGCAGCGATTGAAGAAGACGATATTCTCGCATTCTTTGATACGGAAATCGGACAAAAGCTTCTCGGC
GCCCGCCGCGTCGAACGGGAAGTTCCCTTTAACATGACGCTGTCCGCCAAAGAGGTTTATCCGGATCTTGAGTCAGCCGA
CGAACCTGTGTTGATACAGGGAATTATCGATTGTTTATTCGAGACGGAGGACGGTTTTTACCTTCTTGATTACAAAACTG
ACCGGATACACGGAAAGTACCGAAACGGCTTCGAAGGGGCCGAACCGATTTTAAGGAAGCGTTACGAAACACAAATTCAG
CTTTATGCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGTGCACTGTACTTTTTTGACGGAGGACACGT
GCTCTTATTTTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1051-1222)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	71.893	100	0.72