Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OPR71_RS01570 Genome accession   NZ_CP110670
Coordinates   336125..337141 (+) Length   338 a.a.
NCBI ID   WP_005382774.1    Uniprot ID   A0AAP5Z0M3
Organism   Vibrio alginolyticus strain AUSMDU00064140     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331125..342141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OPR71_RS01555 (OPR71_01555) - 331556..332284 (-) 729 WP_054866559.1 redoxin family protein -
  OPR71_RS01560 (OPR71_01560) oxyR 332454..333365 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  OPR71_RS01565 (OPR71_01565) - 333419..335980 (-) 2562 WP_025768081.1 penicillin-binding protein 1A -
  OPR71_RS01570 (OPR71_01570) pilM 336125..337141 (+) 1017 WP_005382774.1 type IV pilus biogenesis protein PilM Machinery gene
  OPR71_RS01575 (OPR71_01575) pilN 337125..337700 (+) 576 WP_167415488.1 PilN domain-containing protein Machinery gene
  OPR71_RS01580 (OPR71_01580) pilO 337693..338286 (+) 594 WP_005381322.1 type 4a pilus biogenesis protein PilO Machinery gene
  OPR71_RS01585 (OPR71_01585) pilP 338276..338791 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  OPR71_RS01590 (OPR71_01590) pilQ 338817..340562 (+) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  OPR71_RS01595 (OPR71_01595) aroK 340750..341268 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37609.97 Da        Isoelectric Point: 7.2224

>NTDB_id=755890 OPR71_RS01570 WP_005382774.1 336125..337141(+) (pilM) [Vibrio alginolyticus strain AUSMDU00064140]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGVNSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=755890 OPR71_RS01570 WP_005382774.1 336125..337141(+) (pilM) [Vibrio alginolyticus strain AUSMDU00064140]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATTTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTTGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGTAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTGGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

83.136

100

0.831
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503