Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OKX00_RS08155 Genome accession   NZ_CP110666
Coordinates   1598840..1599880 (+) Length   346 a.a.
NCBI ID   WP_265410499.1    Uniprot ID   -
Organism   Fictibacillus sp. KU28468     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1593840..1604880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OKX00_RS08140 (OKX00_08140) - 1594985..1595716 (+) 732 WP_265410064.1 PRC-barrel domain-containing protein -
  OKX00_RS08145 (OKX00_08145) - 1595733..1597160 (+) 1428 WP_265410065.1 G5 domain-containing protein -
  OKX00_RS08150 (OKX00_08150) - 1597166..1598830 (+) 1665 WP_265410498.1 GspE/PulE family protein -
  OKX00_RS08155 (OKX00_08155) pilT 1598840..1599880 (+) 1041 WP_265410499.1 type IV pilus twitching motility protein PilT Machinery gene
  OKX00_RS08160 (OKX00_08160) - 1599882..1601093 (+) 1212 WP_265410066.1 type II secretion system F family protein -
  OKX00_RS08165 (OKX00_08165) - 1601154..1601630 (+) 477 WP_265410067.1 type II secretion system protein -
  OKX00_RS08170 (OKX00_08170) - 1601696..1602451 (+) 756 WP_265410500.1 prepilin peptidase -
  OKX00_RS08175 (OKX00_08175) pilM 1602480..1603478 (+) 999 WP_265410068.1 type IV pilus biogenesis protein PilM -
  OKX00_RS08180 (OKX00_08180) - 1603468..1604061 (+) 594 WP_265410069.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38716.46 Da        Isoelectric Point: 7.9871

>NTDB_id=755863 OKX00_RS08155 WP_265410499.1 1598840..1599880(+) (pilT) [Fictibacillus sp. KU28468]
MKGKLELWLRAAFQLRASDVHLTVGMPPVFRINGDLKKYGNEPLLPEDTYGIARSIIPSESYETFKEKGELDFSYGIKGV
SRFRINTYHQRSCVSLAIRVIPTSIPTLEDLALPDTLKKMAVKPQGLILVTGPTGSGKSTTQASIIDYMNRTMRRHIITL
EDPIEYLHRHNTCIIDQREVGFDTANFANGLRSSLRQDPDVILLGEMRDLETISTAITAAETGHLVLGTLHTSSAPSTID
RIIDVFPPNQQGQIRIQLASVLVGILSQRLFPTPDKNGRKAVTEILLNNSAVANLIRNEKIHQIHNVMQTSKASGMHTFE
MSLKEALERQEILKESAEPYLQERSY

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=755863 OKX00_RS08155 WP_265410499.1 1598840..1599880(+) (pilT) [Fictibacillus sp. KU28468]
ATGAAAGGAAAATTGGAATTATGGCTGAGGGCAGCTTTTCAGCTGAGAGCATCGGATGTTCACTTAACCGTTGGCATGCC
GCCGGTTTTCCGGATTAATGGTGATTTAAAAAAATACGGAAACGAGCCGCTGCTGCCGGAAGACACGTACGGGATTGCCC
GGTCGATTATTCCTTCTGAAAGCTATGAAACATTCAAAGAAAAGGGAGAACTTGATTTTTCCTACGGCATAAAAGGAGTA
TCCCGTTTCAGGATCAATACATACCATCAGCGGTCGTGTGTCAGTCTGGCGATCCGGGTTATTCCTACAAGCATCCCGAC
TCTGGAGGATCTTGCCCTTCCTGATACGTTAAAAAAGATGGCAGTAAAGCCGCAAGGGCTGATCCTGGTAACAGGGCCGA
CGGGAAGCGGGAAATCGACCACTCAAGCTTCTATCATTGATTATATGAACCGCACGATGCGACGCCATATCATCACACTT
GAAGACCCGATTGAGTATTTGCACCGGCACAATACGTGCATCATCGACCAGCGTGAAGTCGGGTTTGATACGGCTAACTT
TGCTAACGGGCTTCGTTCATCGCTCAGACAGGATCCTGATGTCATTCTGCTGGGGGAAATGAGAGATTTGGAGACGATCT
CAACAGCCATAACAGCTGCGGAAACGGGACATTTGGTGTTAGGAACGCTTCATACAAGCAGTGCACCGTCCACGATCGAC
CGGATCATAGACGTGTTTCCGCCGAATCAGCAGGGGCAGATCCGCATTCAGCTGGCTTCCGTGCTTGTCGGTATTCTTTC
ACAGCGTTTGTTCCCAACTCCTGATAAGAATGGAAGAAAAGCCGTAACAGAAATTCTTTTAAACAACTCTGCAGTTGCCA
ACTTGATCCGCAATGAGAAAATTCACCAGATCCATAATGTCATGCAGACGAGCAAGGCATCGGGCATGCATACCTTTGAG
ATGTCGCTGAAGGAAGCGTTGGAACGGCAGGAAATATTAAAAGAGTCGGCTGAGCCTTACCTTCAGGAGAGGAGTTACTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(82-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.852

93.642

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.201

90.462

0.445
  pilT Vibrio cholerae strain A1552

49.201

90.462

0.445
  pilT Legionella pneumophila strain Lp02

46.894

93.064

0.436
  pilT Legionella pneumophila strain ERS1305867

46.894

93.064

0.436
  pilT Neisseria meningitidis 8013

46.855

91.908

0.431
  pilT Neisseria gonorrhoeae MS11

45.963

93.064

0.428
  pilT Acinetobacter baumannii strain A118

45.122

94.798

0.428
  pilT Acinetobacter baumannii D1279779

45.122

94.798

0.428
  pilT Acinetobacter nosocomialis M2

45.122

94.798

0.428
  pilT Pseudomonas aeruginosa PAK

45.171

92.775

0.419
  pilT Pseudomonas stutzeri DSM 10701

44.72

93.064

0.416
  pilT Acinetobacter baylyi ADP1

44.72

93.064

0.416
  pilU Pseudomonas stutzeri DSM 10701

42.216

96.532

0.408
  pilU Vibrio cholerae strain A1552

38.922

96.532

0.376
  pilU Acinetobacter baylyi ADP1

39.937

91.908

0.367