NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	ONZ60_RS20210	Genome accession	NZ_CP110650
Coordinates	4242668..4243543 (+)	Length	291 a.a.
NCBI ID	WP_005314162.1	Uniprot ID	A2T195
Organism	Aeromonas salmonicida strain BG
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4237668..4248543

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ONZ60_RS20190 (ONZ60_20200)	nadC	4237905..4238762 (+)	858	WP_005314155.1	carboxylating nicotinate-nucleotide diphosphorylase	-
ONZ60_RS20195 (ONZ60_20205)	tapA	4239097..4239525 (+)	429	WP_005314158.1	type IVa pilus major pilin TapA	-
ONZ60_RS20200 (ONZ60_20210)	pilB	4239529..4241235 (+)	1707	WP_005314160.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
ONZ60_RS20205 (ONZ60_20215)	-	4241616..4242604 (+)	989	Protein_3918	type II secretion system F family protein	-
ONZ60_RS20210 (ONZ60_20220)	pilD	4242668..4243543 (+)	876	WP_005314162.1	prepilin peptidase	Machinery gene
ONZ60_RS20215 (ONZ60_20225)	coaE	4243563..4244177 (+)	615	WP_005314164.1	dephospho-CoA kinase	-
ONZ60_RS20220 (ONZ60_20230)	zapD	4244215..4244937 (+)	723	WP_005314166.1	cell division protein ZapD	-
ONZ60_RS20225 (ONZ60_20235)	yacG	4244947..4245141 (+)	195	WP_005314167.1	DNA gyrase inhibitor YacG	-
ONZ60_RS20230 (ONZ60_20240)	mutT	4245218..4245625 (-)	408	WP_005314168.1	8-oxo-dGTP diphosphatase MutT	-
ONZ60_RS20235 (ONZ60_20245)	-	4245634..4246806 (-)	1173	WP_005314169.1	tetratricopeptide repeat protein	-

Sequence

Protein

Download Length: 291 a.a. Molecular weight: 32500.78 Da Isoelectric Point: 6.9383

                                >NTDB_id=755712 ONZ60_RS20210 WP_005314162.1 4242668..4243543(+) (pilD) [Aeromonas salmonicida strain BG]

MTLLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFSSDTPQPEDDERYNLMVPRSCCPRCNH

PITALENIPLLSWLWLKGRCRGCQAAISARYPLVELLTALLSVVVAMTLTPGWGTLAALLLTWVLVALTFIDLDKMLLPD

QLTLPLLWGGLLFNLLGGYVPLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSS

LVGAIFGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLSTIL

Nucleotide

Download Length: 876 bp

                                >NTDB_id=755712 ONZ60_RS20210 WP_005314162.1 4242668..4243543(+) (pilD) [Aeromonas salmonicida strain BG]

ATGACACTTCTTCTCGAGCTGGCACACGGCTTGCCATGGCTCTACTTCTCGCTGGTTTTTCTCTTCTCCCTGATGATCGG

CAGCTTTCTCAACGTGGTGATCCACCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCGCAGCTATTTCA

GTTCAGACACGCCGCAGCCAGAAGATGACGAGCGCTACAATCTGATGGTGCCGCGCTCCTGCTGCCCTCGCTGCAATCAC

CCCATAACGGCCTTGGAAAACATTCCCCTGCTGAGCTGGCTCTGGCTCAAAGGTCGCTGCCGGGGCTGCCAGGCCGCCAT

TTCGGCTCGCTATCCGCTGGTGGAGCTGCTGACCGCGCTGCTCTCTGTGGTGGTTGCCATGACGCTCACCCCTGGCTGGG

GTACGCTGGCTGCCCTCCTGCTGACCTGGGTGCTGGTGGCGCTCACCTTTATCGATCTGGACAAGATGCTGCTGCCGGAC

CAGCTCACCTTGCCGCTACTGTGGGGCGGGCTGCTGTTCAACCTGCTGGGCGGATACGTGCCTCTCGGCGATGCTGTCAT

CGGCGCCATGGCAGGCTATCTGGTGCTGTGGAGCCTCTACTGGGCCTTCAAGCTGCTTACCGGCAAGGAGGGCATGGGCT

ATGGGGACTTCAAACTGCTGGCAGCGCTGGGAGCCTGGCTTGGCTGGCAGGCGCTGCCCATAGTACTGCTGCTCTCTTCT

CTGGTCGGCGCCATCTTCGGCATTGGCCTCATTTTGCTGCGCAACCATCACCAGAGCAAACCCATCCCCTTTGGCCCCTA

TCTCGCCATCGCAGGCTGGATCGCACTGCTCTGGGGCGATAGCATCACCCGCTGGTATCTCTCCACTATCCTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(140-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A2T195

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio cholerae strain A1552	60.764	98.969	0.601
pilD	Vibrio campbellii strain DS40M4	55.556	98.969	0.55
pilD	Acinetobacter baumannii D1279779	55.39	92.44	0.512
pilD	Acinetobacter nosocomialis M2	55.39	92.44	0.512
pilD	Neisseria gonorrhoeae MS11	50.746	92.096	0.467

Detailed information NTG0755712