Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ONR73_RS02920 Genome accession   NZ_CP110364
Coordinates   595857..596966 (+) Length   369 a.a.
NCBI ID   WP_058057996.1    Uniprot ID   -
Organism   Aeromonas veronii strain C4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 590857..601966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONR73_RS02890 (ONR73_02890) - 591758..592177 (-) 420 WP_157161459.1 DUF4426 domain-containing protein -
  ONR73_RS02895 (ONR73_02895) yggU 592221..592523 (-) 303 WP_005340341.1 DUF167 family protein YggU -
  ONR73_RS02900 (ONR73_02900) - 592523..593074 (-) 552 WP_005340343.1 YggT family protein -
  ONR73_RS02905 (ONR73_02905) proC 593086..593910 (-) 825 WP_265062073.1 pyrroline-5-carboxylate reductase -
  ONR73_RS02910 (ONR73_02910) - 594053..594754 (-) 702 WP_128815094.1 YggS family pyridoxal phosphate-dependent enzyme -
  ONR73_RS02915 (ONR73_02915) pilT 594794..595828 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  ONR73_RS02920 (ONR73_02920) pilU 595857..596966 (+) 1110 WP_058057996.1 type IVa pilus ATPase TapU Machinery gene
  ONR73_RS02925 (ONR73_02925) yaaA 597014..597787 (+) 774 WP_064337358.1 peroxide stress protein YaaA -
  ONR73_RS02930 (ONR73_02930) - 597982..598407 (+) 426 WP_005340360.1 hypothetical protein -
  ONR73_RS02935 (ONR73_02935) - 598766..599257 (-) 492 WP_139747064.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  ONR73_RS02940 (ONR73_02940) arfB 599283..599699 (-) 417 WP_265062074.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  ONR73_RS02945 (ONR73_02945) glnK 599901..600239 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  ONR73_RS02950 (ONR73_02950) - 600498..601505 (+) 1008 WP_265062880.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41053.08 Da        Isoelectric Point: 6.4327

>NTDB_id=754559 ONR73_RS02920 WP_058057996.1 595857..596966(+) (pilU) [Aeromonas veronii strain C4]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALANADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=754559 ONR73_RS02920 WP_058057996.1 595857..596966(+) (pilU) [Aeromonas veronii strain C4]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAGGCGCTTGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGTCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCTACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGCCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGTAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGCTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTGCCGCAAGACAAGCACCGCCAGTTCCTGTTCGATCTTTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCAAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATCA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACATTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCAACGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGTACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.114

95.122

0.572
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Legionella pneumophila strain Lp02

41.667

87.805

0.366
  pilT Legionella pneumophila strain ERS1305867

41.667

87.805

0.366
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36