Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OL445_RS05550 Genome accession   NZ_CP110362
Coordinates   1227090..1228103 (-) Length   337 a.a.
NCBI ID   WP_284603487.1    Uniprot ID   -
Organism   Dickeya dadantii strain XJ12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1222090..1233103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OL445_RS05530 - 1223835..1224746 (+) 912 WP_284603484.1 helix-turn-helix domain-containing protein -
  OL445_RS05535 codA 1224749..1225999 (+) 1251 WP_284603485.1 cytosine deaminase -
  OL445_RS05540 - 1226154..1226774 (+) 621 WP_284603486.1 Fic family protein -
  OL445_RS05545 - 1226753..1226944 (-) 192 WP_033112044.1 hypothetical protein -
  OL445_RS05550 pilT 1227090..1228103 (-) 1014 WP_284603487.1 type IV pilus twitching motility protein PilT Machinery gene
  OL445_RS05555 - 1228125..1228859 (+) 735 WP_284603488.1 YggS family pyridoxal phosphate-dependent enzyme -
  OL445_RS05560 proC 1228893..1229714 (+) 822 WP_284603489.1 pyrroline-5-carboxylate reductase -
  OL445_RS05565 - 1229758..1230312 (+) 555 WP_013319314.1 YggT family protein -
  OL445_RS05570 yggU 1230309..1230599 (+) 291 WP_033112045.1 DUF167 family protein YggU -
  OL445_RS05575 - 1230646..1231239 (+) 594 WP_284603490.1 XTP/dITP diphosphatase -
  OL445_RS05580 hemW 1231232..1232371 (+) 1140 WP_284603491.1 radical SAM family heme chaperone HemW -
  OL445_RS05585 - 1232496..1232624 (+) 129 WP_013319318.1 entericidin A/B family lipoprotein -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 36911.12 Da        Isoelectric Point: 6.6323

>NTDB_id=754523 OL445_RS05550 WP_284603487.1 1227090..1228103(-) (pilT) [Dickeya dadantii strain XJ12]
MDVSEWVRSSVKQNVSDLHLCSGHPPIWRIDGQLVADAAQPPLHNAELEQWCHSWLEPACEQQLRQSGQTDCALTLPEGQ
RLRANLFMQRHGLSAALRLIPSHQPDLRTLGAPPVVAELTERADGLILVTGATGSGKSTTLAAMIAALNQRSARHIITLE
DPIEFLHTSQRSLIQQREIGRHSEGFASALRAALREDPDVLLLGELRDNETIRLALTAAETGHLVLATLHTRRAVQAVDR
LVDVFPAEEKAFVRAQLAASLQAVVAQKLLPGVQGGRVALFEVLLATPAVSNLIREGKTHQVPSLLQTGSQVGMQTFEQS
YQQRCREGMLRPEINAD

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=754523 OL445_RS05550 WP_284603487.1 1227090..1228103(-) (pilT) [Dickeya dadantii strain XJ12]
ATGGATGTGAGTGAATGGGTGCGGTCTAGTGTAAAACAGAATGTGTCCGATCTGCACCTGTGCAGCGGTCATCCGCCGAT
TTGGCGTATCGACGGGCAACTGGTGGCGGATGCCGCGCAGCCGCCGTTGCACAACGCCGAACTGGAACAGTGGTGCCACA
GTTGGCTGGAGCCGGCCTGCGAGCAGCAGCTACGGCAAAGCGGGCAGACCGATTGCGCGCTGACGCTGCCGGAGGGCCAG
CGGTTACGGGCTAATCTGTTTATGCAGCGCCACGGCCTGTCGGCGGCGTTACGTCTGATCCCGTCACATCAACCCGACCT
GCGAACGCTGGGCGCGCCGCCGGTGGTGGCGGAGCTGACCGAGCGCGCCGATGGCCTGATTCTGGTGACCGGCGCCACCG
GCAGCGGTAAATCCACCACCCTGGCGGCGATGATTGCCGCGCTTAATCAACGCAGCGCACGCCATATCATTACGCTGGAA
GACCCGATTGAGTTCCTGCATACCAGCCAGCGCAGCCTGATCCAGCAGCGGGAGATCGGCCGTCACAGCGAAGGATTCGC
CAGCGCGCTGCGGGCGGCGCTGCGCGAAGACCCGGATGTGCTGCTGCTCGGCGAGTTGCGCGACAACGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGCTGGCGACGCTGCATACCCGGCGGGCGGTGCAGGCGGTGGATCGG
CTGGTGGACGTTTTTCCCGCCGAGGAGAAAGCGTTTGTCCGCGCGCAACTGGCCGCCAGCCTGCAGGCGGTGGTGGCGCA
GAAACTGTTGCCGGGCGTGCAAGGCGGGCGTGTGGCGCTGTTTGAAGTGCTGCTCGCCACCCCGGCGGTCAGCAACCTGA
TCCGCGAAGGCAAAACCCACCAGGTGCCCAGCCTGCTGCAAACCGGCAGCCAGGTCGGTATGCAGACTTTTGAACAGAGT
TACCAGCAGCGGTGCCGGGAGGGGATGCTGCGGCCTGAGATCAATGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.697

97.923

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.697

97.923

0.487
  pilT Neisseria meningitidis 8013

48.955

99.407

0.487
  pilT Neisseria gonorrhoeae MS11

48.802

99.11

0.484
  pilT Pseudomonas stutzeri DSM 10701

49.091

97.923

0.481
  pilT Pseudomonas aeruginosa PAK

47.748

98.813

0.472
  pilT Acinetobacter baylyi ADP1

47.576

97.923

0.466
  pilT Acinetobacter baumannii D1279779

47.147

98.813

0.466
  pilT Acinetobacter nosocomialis M2

47.147

98.813

0.466
  pilT Acinetobacter baumannii strain A118

47.147

98.813

0.466
  pilT Legionella pneumophila strain ERS1305867

46.97

97.923

0.46
  pilT Legionella pneumophila strain Lp02

46.97

97.923

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.828

94.659

0.424
  pilU Pseudomonas stutzeri DSM 10701

37.425

99.11

0.371
  pilU Vibrio cholerae strain A1552

38.58

96.142

0.371