Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PALA47_RS24415 Genome accession   NZ_CP110352
Coordinates   5275680..5276552 (+) Length   290 a.a.
NCBI ID   WP_003107301.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5270680..5281552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA47_RS24390 - 5270891..5271205 (-) 315 WP_269972583.1 pilin -
  PALA47_RS24395 (PALA47_04832) - 5271276..5272256 (-) 981 WP_003117291.1 IS5-like element ISPa41 family transposase -
  PALA47_RS24400 (PALA47_04833) - 5272348..5272527 (-) 180 Protein_4832 pilin -
  PALA47_RS24405 (PALA47_04834) pilB 5272758..5274458 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA47_RS24410 (PALA47_04835) pilC 5274462..5275679 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  PALA47_RS24415 (PALA47_04836) pilD 5275680..5276552 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA47_RS24420 (PALA47_04837) coaE 5276549..5277160 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA47_RS24425 (PALA47_04838) yacG 5277157..5277357 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PALA47_RS24430 (PALA47_04839) - 5277394..5277603 (-) 210 WP_020750398.1 hypothetical protein -
  PALA47_RS24435 (PALA47_04840) - 5277709..5278398 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  PALA47_RS24440 (PALA47_04841) - 5278395..5278865 (-) 471 WP_003103866.1 hypothetical protein -
  PALA47_RS24445 (PALA47_04842) - 5278862..5279287 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  PALA47_RS24450 (PALA47_04843) - 5279420..5280049 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  PALA47_RS24455 (PALA47_04844) - 5280046..5280495 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  PALA47_RS24460 (PALA47_04845) - 5280521..5280691 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31873.90 Da        Isoelectric Point: 7.7068

>NTDB_id=754442 PALA47_RS24415 WP_003107301.1 5275680..5276552(+) (pilD) [Pseudomonas aeruginosa strain PALA47]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=754442 PALA47_RS24415 WP_003107301.1 5275680..5276552(+) (pilD) [Pseudomonas aeruginosa strain PALA47]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.357

96.552

0.534
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.921

95.862

0.469