Detailed information NTG0753647

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: OM992_RS05950
• Genome accession: NZ_CP110268
• Coordinates: 1129181..1132885 (+)
• Length: 1234 a.a.
• NCBI ID: WP_264820292.1
• Uniprot ID: -
• Organism: Bacillus siamensis strain YB-1631
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1124181..1137885

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OM992_RS05935 (OM992_05935)	-	1124261..1124770 (-)	510	WP_049628231.1	ferritin-like domain-containing protein	-
OM992_RS05940 (OM992_05940)	-	1124839..1125552 (-)	714	WP_016938292.1	DUF421 domain-containing protein	-
OM992_RS05945 (OM992_05945)	addB	1125694..1129194 (+)	3501	WP_264819953.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
OM992_RS05950 (OM992_05950)	addA	1129181..1132885 (+)	3705	WP_264820292.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
OM992_RS05955 (OM992_05955)	sbcD	1132951..1134123 (+)	1173	WP_264819954.1	exonuclease subunit SbcD	-
OM992_RS05960 (OM992_05960)	sbcC	1134120..1137512 (+)	3393	WP_264819955.1	exonuclease subunit SbcC	-
OM992_RS05965 (OM992_05965)	-	1137529..1137828 (+)	300	WP_264819956.1	HNH endonuclease	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141409.78 Da
• Isoelectric Point: 5.1601

>NTDB_id=753647 OM992_RS05950 WP_264820292.1 1129181..1132885(+) (addA) [Bacillus siamensis strain YB-1631]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGSQA
FFELVDRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADNFLDDLQQIEELISCRHDFEALYERVPAVTFKRAKAVKGDEFDKALLDEATDLRNSAKKLIEKLK
TDYFTRSPQDHLKSLTDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAADEDGRRVPSEAAVYYQEQ
FHEVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNRNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNEAAETELLLIDSADDADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDELYQKLRTFYDNLQKWRSFSTNHSVAELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKNREKQLAKWQAQADRADWLLSEFDRYQATSYLDFIGPALIRHRDMEAHRPPGLSAPEDIAHDPSRFHIRMLQQSE
LLEDNPKERAEEKSKRLKAIQQGEPIPDSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYRRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRTLHEKELLTEEQWDAIDIDDIIQFFETEIGK
DLLGARRIDREVPFSMALPAGEVYKDAKTAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAIEQITKTKVKGRALYFFDGGHVLTL

Nucleotide

• Length: 3705 bp

>NTDB_id=753647 OM992_RS05950 WP_264820292.1 1129181..1132885(+) (addA) [Bacillus siamensis strain YB-1631]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACCGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAGGAAGATC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATCGCTGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTCCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACGCTGCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGCTTTCGGATGGCTG
ATCAGACGGAAGGCGAATTGCTCGGAGACGAAGTCCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAGTCAGGCG
TTTTTTGAGCTTGTGGACAGATATACGACGGACAGGCATGATTTGGATCTTCAAGATCTCGTCAAGCGGGTGTACGAGTA
TTCACGGTCTCATCCTGATCCGGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACGGAAGAAAGCAAAATGG
AAGAGCTGCCGTTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAGCAAAAGCTTGAAAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCGCCGCGCGCTGATAATTTTCTGGATGATCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTTGAGGCGCTGTATGAACGGGTTCCCGCGGTCACATTTAAGCGGGCTAAGGCGGTAAAAG
GTGATGAATTTGACAAGGCGCTTTTGGATGAAGCGACCGATTTGCGAAACAGCGCGAAAAAACTGATCGAAAAGTTAAAG
ACCGATTACTTTACAAGAAGCCCGCAAGATCATTTAAAGAGTTTAACAGACATGAAGCCTGTCATTGAAACGCTCGTTCA
GCTCGTGATTTCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGTCTGGCGATTTTAACTGCGGCTGATGAAGACGGCCGGCGGGTGCCGAGTGAAGCGGCAGTTTATTATCAAGAGCAG
TTCCATGAGGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAATCTATTCTGCAGCTTGTGAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGACGTCAAACAATCGATCTACCGTTTCAGACTTGCCGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAGAGCGGGGAAGGAGCGGGACAGAAAATTGACCTGAACCGAAACTTCCGCAGC
CGCTCCGATATTTTGGACAGCACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTGAAGCTCGGTGCGTCGTATCCGCCGAATGAGGCGGCGGAAACGGAACTGCTGCTGATTGACAGTGCTG
ATGATGCAGACAGCTCCGAGGATGCCGAAGATTTTGAAACCGTGCATTGGGAAGCAAAAGCGATAGCCGGGGAGATCCGG
AAGCTTGTCTCATCTCCGTTTAAGGTCTATGACGGCAAAACCAAAACACACCGGAATATCCAGTACCGGGATATCGTGAT
TTTGCTGCGTTCCATGCCATGGGCGCCGCAATTGATGGAAGAACTGAAAAACCAGGGCATTCCGGTTTACGCCAATTTGA
CGTCAGGTTATTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTAAAGGTGATTGACAACCCGTATCAGGATATT
CCGCTTGCTTCCGTACTGCGCTCGCCTATTGTCGGCTGTGACGAAAATGAACTTGCGCTGATACGCCTGGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGGTGACAGACATGACGAGCTGTATCAAAAGCTGAGAACAT
TTTATGACAATCTGCAAAAGTGGCGGTCATTCTCGACGAATCATTCGGTAGCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGATATTTTGATTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATCTGGGGACGGCGCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATTCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTGCCGCTTGTGGCGATGAAGAAAA
AAATGCGCAGAGAGCTTTTATCAGAAGAATTGCGCGTGTTATATGTGGCGCTGACAAGAGCGAAGGAAAAGCTGTTTCTC
ATCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCGGACAGGGCCGATTGGCTTCTCTCTGA
ATTCGACCGCTATCAGGCGACATCTTATTTGGACTTTATCGGGCCGGCGCTTATCCGCCACCGTGACATGGAGGCGCACA
GGCCGCCGGGTCTTTCGGCTCCGGAGGATATCGCGCACGACCCGTCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAA
CTGCTGGAAGATAACCCAAAAGAACGGGCTGAAGAAAAGAGCAAACGTCTCAAGGCGATTCAGCAGGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTCCTTGAATGGGAATACCCGTACCGAGAGCTGACGGCTATCAGAA
CGAAGCAGTCAGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCCCTCATCAAACCGTCA
GGGGACACGCTTTTATACAGACGTCCCGGTTTTATGATGAAAAAGGGGCTGACGGCCGCGGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCACGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTTCGGACGCTTCACGAAA
AAGAGCTGCTGACAGAAGAGCAGTGGGACGCTATTGATATCGACGACATTATACAGTTTTTCGAAACTGAAATCGGGAAA
GACCTTCTCGGGGCGCGGCGGATTGATCGGGAGGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGC
AAAAACGGCCGGTGAACCGCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACGGCGGACGGACTCTATTTGCTGG
ACTATAAAACAGATCGGATAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCCCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGATTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1226)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-882)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	82.496	100	0.825