Detailed information NTG0753646

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: OM992_RS05945
• Genome accession: NZ_CP110268
• Coordinates: 1125694..1129194 (+)
• Length: 1166 a.a.
• NCBI ID: WP_264819953.1
• Uniprot ID: -
• Organism: Bacillus siamensis strain YB-1631
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1120694..1134194

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OM992_RS05920 (OM992_05920)	-	1120893..1122050 (-)	1158	WP_264819951.1	AbrB family transcriptional regulator	-
OM992_RS05925 (OM992_05925)	-	1122216..1123412 (-)	1197	WP_016938295.1	MFS transporter	-
OM992_RS05930 (OM992_05930)	-	1123443..1124051 (-)	609	WP_264819952.1	TetR/AcrR family transcriptional regulator	-
OM992_RS05935 (OM992_05935)	-	1124261..1124770 (-)	510	WP_049628231.1	ferritin-like domain-containing protein	-
OM992_RS05940 (OM992_05940)	-	1124839..1125552 (-)	714	WP_016938292.1	DUF421 domain-containing protein	-
OM992_RS05945 (OM992_05945)	addB	1125694..1129194 (+)	3501	WP_264819953.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
OM992_RS05950 (OM992_05950)	addA	1129181..1132885 (+)	3705	WP_264820292.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
OM992_RS05955 (OM992_05955)	sbcD	1132951..1134123 (+)	1173	WP_264819954.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 133580.32 Da
• Isoelectric Point: 5.4535

>NTDB_id=753646 OM992_RS05945 WP_264819953.1 1125694..1129194(+) (addB) [Bacillus siamensis strain YB-1631]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQLPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SGEQAPDELDLFRMTGSTYYKLYQMAKELNADISCKELHGTKRHQHVPELACIESQYDVRPAAAYTGRQEAFTVIQAQNR
RAELEGIAREIQSLVRDGGYRYRDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPVQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEQMLNETREW
MAAPLVRLQNRMKKAKTVKSMAEALYLFLEDTDVPLKLDRKRQQAEETGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEM
PLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLRAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPEHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHRRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPRLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGNGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSEQDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide

• Length: 3501 bp

>NTDB_id=753646 OM992_RS05945 WP_264819953.1 1125694..1129194(+) (addB) [Bacillus siamensis strain YB-1631]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGA
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTGTTGAGGAAGCTGATTGAAGAGCATAAGCACGATTT
TAAAGTGTATCAAAAAGCGAGTGATAAAACCGGGTTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGTTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGGGAGCGGATGCTGTCTGAA
AAACTTCATGACTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGCCACTATCTGCATTCAGAGGATTATTT
GACGCTGCTCGCCCAGCAGCTTCCATTAGCGGATGTTGTAAAAGGCGCCAGAGTTTACGTCGACGGCTTCTATCAATTTA
CACCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGTCCT
TCGGGAGAACAGGCACCGGATGAGCTCGATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGATGGCAAA
GGAATTGAATGCCGATATTTCCTGCAAGGAACTGCATGGCACGAAACGGCATCAGCATGTGCCGGAACTTGCCTGCATAG
AGTCGCAATACGATGTGCGTCCCGCTGCGGCGTATACCGGCCGGCAGGAAGCGTTTACCGTCATACAGGCGCAAAACAGA
AGAGCCGAGCTTGAAGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACGGAGGATACCGGTACCGGGATATGGCGAT
TCTCATCCGCCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCAGATTACGGGCTTCCTTATTTTATTGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATAGAATTCATCCGTTCAAGTTTGGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGTGCAGAAAATAAGAGAGCAGGTCGA
TCAGCTTGAAAACTATTGTATCGCCTACGGAATTAAAGGGGAGCGCTGGACGAACGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAGCAAATGCTGAATGAGACGCGAGAATGG
ATGGCCGCGCCCCTCGTCCGGCTTCAAAACCGGATGAAAAAAGCGAAAACGGTTAAAAGCATGGCGGAAGCTCTTTATCT
CTTTTTGGAAGACACCGATGTTCCCCTGAAGCTGGACCGGAAAAGGCAGCAGGCCGAAGAGACCGGAAACATGATTGAAG
CGCAGCAGCACGGCCAGGCATGGGACGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATG
CCTTTGGCGCTGTTCCAGCAAATGCTTGAAACAGGAACGGAATCACTTCATTTTTCTTTAATTCCCCCTGCGCTTGACCA
AGTGTTTGTAGGGAATATGGACCTGTCAAGAATGTACGGCACGTCCTGCACGTTTGTCATCGGGGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCCGATGATGACCGGGAGTGGCTGAGAGCGGTCGGAGTGGAGCTGTCT
TCCGCGGGGCGTGAGCGCCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTGTATGT
GTCATACCCGATTGCCGATGCGGAAGGAAAAACGCTTCTGCCCTCAATTGTTGTAAACAGGCTTGGCGAGCTGTTTCCCG
AACATCAGGAAAAACTGTCGGCTGCTGATCCCGAGCAGGTAAGTGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCGCAGACGTATACGGCAAGCCAGCTCAGGCTGTGGACAAGAGAGTATGAAATCAGTGATGTATGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGCCTGATCACCGGCGCGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGCAATGAGGCGAAGC
GTCTTGAACGTCCTGTTTCGCGCCAGCTTTACGGCGAGCACATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCCCATTTTGCATCTCACGGGCTTCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGAAGCGCCCGATATCGG
ACAGCTGTTTCACTCCAGCCTCAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAGGCTGCAAAAAGAAATTCTGCTCAGTTCAAAC
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTCTCTGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTCGTCCCGATCGGCCTTGAACTCGGCTTCGGGGGAAACGGTCCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGCCGGATCGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGCCTGCTGCTTAGAATCGTG
GATTACAAATCAAGCGACAAGGGGCTTGATCTGGCTGAGGTATATTACGGGCTCGCTCTGCAAATGCTCACGTACCTTGA
CTTATCGATTACACATTCAGCGGATTGGCTCGGCATGAAAGCGACACCCGCAGGCGTTTTGTATTTCCATATCCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTCAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGATCGGGAGGCAATCAGCCTGATGGACACGACTTTGGAGGAAGGACGTTCAAATATCATTAATGCAGGTTT
GAAAAAGGACGGTTCTTTAAGATCCGATTCAGCCGCAGTCAGCGAACAGGATTTTCATCTCCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAATCGGTGTGCCAGTTTGATGAGTCTCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (791-1131)

• UvrD-like helicase C-terminal domain (UvrD_C) (310-669)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	83.019	100	0.83