Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   UKS_RS00135 Genome accession   NZ_AP021887
Coordinates   25652..27013 (+) Length   453 a.a.
NCBI ID   WP_156011312.1    Uniprot ID   -
Organism   Streptococcus sp. 116-D4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20652..32013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UKS_RS00105 (UKS_00170) comW 22071..22307 (+) 237 WP_156011310.1 sigma(X)-activator ComW Regulator
  UKS_RS00110 (UKS_00180) - 22538..23824 (+) 1287 WP_004237599.1 adenylosuccinate synthase -
  UKS_RS00115 (UKS_00190) tadA 24024..24491 (+) 468 WP_156011311.1 tRNA adenosine(34) deaminase TadA -
  UKS_RS00125 (UKS_00200) - 24678..25121 (+) 444 WP_156013004.1 dUTP diphosphatase -
  UKS_RS00130 - 25072..25638 (+) 567 Protein_19 histidine phosphatase family protein -
  UKS_RS00135 (UKS_00220) radA 25652..27013 (+) 1362 WP_156011312.1 DNA repair protein RadA Machinery gene
  UKS_RS00140 (UKS_00230) - 27086..27583 (+) 498 WP_049495678.1 carbonic anhydrase -
  UKS_RS00145 (UKS_00240) - 27608..28426 (+) 819 WP_156011313.1 PrsW family glutamic-type intramembrane protease -
  UKS_RS00150 (UKS_00250) - 28572..29540 (+) 969 WP_156011314.1 ribose-phosphate diphosphokinase -
  UKS_RS00155 (UKS_00260) - 29659..30609 (+) 951 WP_156011315.1 Rpn family recombination-promoting nuclease/putative transposase -
  UKS_RS00160 (UKS_00280) - 30764..30949 (+) 186 WP_156011316.1 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49397.86 Da        Isoelectric Point: 6.3737

>NTDB_id=75317 UKS_RS00135 WP_156011312.1 25652..27013(+) (radA) [Streptococcus sp. 116-D4]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
HIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITPPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=75317 UKS_RS00135 WP_156011312.1 25652..27013(+) (radA) [Streptococcus sp. 116-D4]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGTCCCAACTG
TGGGTCTTGGTCTTCTTTTGTAGAAGAGGTTGAGGTTGCCGAGGTCAAAAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAACCCATGAAACTGGCTGAAGTGACTTCCATCAATGTCAATCGAACCAAGACAGAGATGGAGGAGTTCAATCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTTGTTCTTATCGGTGGGGATCCTGGGATTGGGAAATCAACCCTTCTCTTACA
AGTCTCAACCCAGCTGTCTCAAGTAGGGACAGTTCTCTACGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGTTTGGGAGATATTGATAGCGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGCGCAGAGGTGGAG
CACATCCAGCCAGATTTTCTCATCATCGACTCCATCCAAACCATTATGTCTCCTGAAATTTCAGGGGTGCAGGGGTCTGT
TTCTCAAGTGCGTGAGGTGACAGCGGAGCTCATGCAGCTGGCTAAGACCAATAACATCGCCATCTTTATCGTCGGCCATG
TGACCAAGGAAGGAACCTTGGCGGGTCCGCGTATGTTGGAGCATATGGTGGACACGGTGCTTTACTTTGAAGGGGAACGC
CACCATACCTTCCGTATCTTGAGAGCAGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGCATCTTTGAGATGCAGTC
GGGTGGCTTGGTTGAGGTTCTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGAGCGACTGGCTCGTCAATCG
TTGTGACCATGGAAGGGACGCGTCCGATTTTAGCAGAGGTACAGGCCTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACGACGACAGGTCTTGACTTCAATCGCGCTAGTCTGATTATGGCTGTTTTGGAAAAACGGGCAGGTCTTCTCTTGCA
AAATCAGGATGCTTATCTCAAATCTGCTGGCGGTGTCAAATTGGATGAACCTGCCATTGACTTAGCCGTCGCGGTTGCCA
TTGCTTCGAGTTATAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTAGGAGAATTAGGTTTGACTGGAGAAATTCGG
CGTGTGAATCGTATCGAGCAGCGCATCAATGAAGCTGCTAAACTGGGATTTACAAAAATCTATGTACCTAAGAATTCCTT
GACAGGAATCACTCCGCCTAAGGAAATTCAGGTCATTGGAGTAACAACGATTCAAGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

99.558

100

0.996
  radA Streptococcus mitis SK321

99.558

100

0.996
  radA Streptococcus pneumoniae Rx1

99.558

100

0.996
  radA Streptococcus pneumoniae D39

99.558

100

0.996
  radA Streptococcus pneumoniae R6

99.558

100

0.996
  radA Streptococcus pneumoniae TIGR4

99.558

100

0.996
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment