Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OFY13_RS01310 Genome accession   NZ_CP110188
Coordinates   278030..279046 (+) Length   338 a.a.
NCBI ID   WP_000520446.1    Uniprot ID   -
Organism   Vibrio cholerae strain E1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273030..284046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OFY13_RS01295 (OFY13_01295) - 273571..274302 (-) 732 WP_000495226.1 glutathione peroxidase -
  OFY13_RS01300 (OFY13_01300) oxyR 274442..275335 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  OFY13_RS01305 (OFY13_01305) - 275410..277887 (-) 2478 WP_000668738.1 penicillin-binding protein 1A -
  OFY13_RS01310 (OFY13_01310) pilM 278030..279046 (+) 1017 WP_000520446.1 type IV pilus assembly protein PilM Machinery gene
  OFY13_RS01315 (OFY13_01315) pilN 279036..279620 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  OFY13_RS01320 (OFY13_01320) pilO 279613..280203 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  OFY13_RS01325 (OFY13_01325) pilP 280193..280711 (+) 519 WP_000792030.1 pilus assembly protein PilP Machinery gene
  OFY13_RS01330 (OFY13_01330) pilQ 280737..282452 (+) 1716 WP_000788427.1 type IV pilus secretin PilQ Machinery gene
  OFY13_RS01335 (OFY13_01335) aroK 282639..283163 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37735.45 Da        Isoelectric Point: 7.1127

>NTDB_id=752928 OFY13_RS01310 WP_000520446.1 278030..279046(+) (pilM) [Vibrio cholerae strain E1]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVQVTEKSLVRSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=752928 OFY13_RS01310 WP_000520446.1 278030..279046(+) (pilM) [Vibrio cholerae strain E1]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCCAAGTGACGGAAAAAAGCCTCGTCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACT
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.408

100

0.994
  pilM Vibrio campbellii strain DS40M4

48.225

100

0.482