Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QEO90_RS03815 Genome accession   NZ_CP123311
Coordinates   785179..785988 (+) Length   269 a.a.
NCBI ID   WP_021538963.1    Uniprot ID   -
Organism   Escherichia coli strain AREA_R505     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 780179..790988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEO90_RS03810 (QEO90_03810) sslE 780484..785049 (+) 4566 WP_060624727.1 lipoprotein metalloprotease SslE -
  QEO90_RS03815 (QEO90_03815) pilD 785179..785988 (+) 810 WP_021538963.1 prepilin peptidase PppA Machinery gene
  QEO90_RS03820 (QEO90_03820) gspS2 786054..786464 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  QEO90_RS03825 (QEO90_03825) gspC 786482..787441 (+) 960 WP_024244404.1 type II secretion system protein GspC -
  QEO90_RS03830 (QEO90_03830) gspD 787471..789531 (+) 2061 WP_060624724.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29443.02 Da        Isoelectric Point: 8.3801

>NTDB_id=750180 QEO90_RS03815 WP_021538963.1 785179..785988(+) (pilD) [Escherichia coli strain AREA_R505]
MFFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWSESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=750180 QEO90_RS03815 WP_021538963.1 785179..785988(+) (pilD) [Escherichia coli strain AREA_R505]
ATGTTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATGCCCATACTGGCTACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGTCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTGCCGCGTTCGCACTGTCCGCATTGCCAGCAGACTATCCGCGTTCGTGACAATATTCCG
CTGCTCTCCTGGTTGATGCTCAAAGGGCGCTGCCGTGATTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTTGAGTT
ATTGACAGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGTCAGAAAGTGGATGGGGACTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCATCATCGACCTCGACAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACC
GGGCTGATTGCGGCATGGGCGCAGCAGAGTCCATTAACGCTACAAGACGCAGTCACCGGCGTTCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTTTTCGCCG
CTTTAGGTGGCTGGGTGGGGCCGTTGTCGCTACCCAATGTTGCTTTAATCGCATCATGCTGCGGTCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.944

99.257

0.446
  pilD Vibrio campbellii strain DS40M4

41.697

100

0.42
  pilD Acinetobacter nosocomialis M2

38.491

98.513

0.379
  pilD Neisseria gonorrhoeae MS11

39.3

95.539

0.375
  pilD Acinetobacter baumannii D1279779

38.113

98.513

0.375