Detailed information NTG0749126

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OHK33_RS00170
• Genome accession: NZ_CP109770
• Coordinates: 38696..39541 (+)
• Length: 281 a.a.
• NCBI ID: WP_300993708.1
• Uniprot ID: -
• Organism: Pectobacterium aroidearum strain T25-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 33696..44541

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OHK33_RS00140 (OHK33_00140)	gspI	34089..34454 (+)	366	WP_015841044.1	type II secretion system minor pseudopilin GspI	-
OHK33_RS00145 (OHK33_00145)	gspJ	34454..35104 (+)	651	WP_181829033.1	type II secretion system minor pseudopilin GspJ	-
OHK33_RS00150 (OHK33_00150)	gspK	35101..36087 (+)	987	WP_350316381.1	type II secretion system minor pseudopilin GspK	-
OHK33_RS00155 (OHK33_00155)	gspL	36084..37361 (+)	1278	WP_350316382.1	type II secretion system protein GspL	-
OHK33_RS00160 (OHK33_00160)	-	37358..37852 (+)	495	WP_182099854.1	type II secretion system protein M	-
OHK33_RS00165 (OHK33_00165)	-	37842..38588 (+)	747	WP_015841039.1	type II secretion system protein N	-
OHK33_RS00170 (OHK33_00170)	pilD	38696..39541 (+)	846	WP_300993708.1	A24 family peptidase	Machinery gene
OHK33_RS00175 (OHK33_00175)	ypdK	39684..39758 (-)	75	WP_099606229.1	membrane protein YpdK	-
OHK33_RS00180 (OHK33_00180)	-	40115..41140 (-)	1026	WP_194430533.1	LLM class flavin-dependent oxidoreductase	-
OHK33_RS00185 (OHK33_00185)	-	41616..42680 (+)	1065	WP_350316383.1	ABC transporter substrate-binding protein	-
OHK33_RS00190 (OHK33_00190)	-	42677..43714 (+)	1038	WP_181838176.1	iron ABC transporter permease	-
OHK33_RS00195 (OHK33_00195)	-	43716..44486 (+)	771	WP_320703681.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31275.40 Da
• Isoelectric Point: 7.1428

>NTDB_id=749126 OHK33_RS00170 WP_300993708.1 38696..39541(+) (pilD) [Pectobacterium aroidearum strain T25-1]
MDDLREFALVFPAWWYGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELQTGVTDVEHVTSYNLCWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQPVSVQYPLVEVITMLAFLIAGLLWLPGMALCGALILLSFLVVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSETFVPLRDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGVRKEDTAKPLAFGPWLAIGGVFGVIINGLYL

Nucleotide

• Length: 846 bp

>NTDB_id=749126 OHK33_RS00170 WP_300993708.1 38696..39541(+) (pilD) [Pectobacterium aroidearum strain T25-1]
GTGGACGATTTAAGGGAATTCGCACTGGTATTTCCGGCATGGTGGTATGGTGCGCTGGGCGTATTGGGCTTGATTGTTGG
CAGTTTTCTGAACGTCGTGATTTACCGCCTGCCGATTATGTTAGAACGCCGCTGGCGGCAGGACATAGAGCTGCAAACGG
GTGTGACCGATGTCGAGCATGTGACGAGTTACAACCTATGTTGGCCGCCTTCGTCTTGCCCGCACTGTCAGCAGGCGATT
GCGGTGAAAGATAACATCCCGTTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGCTGCTGTCATCAGCCAGTGTCTGT
GCAGTATCCGCTGGTGGAAGTCATCACGATGCTGGCTTTTCTGATCGCAGGGTTACTGTGGCTGCCCGGCATGGCGTTAT
GCGGGGCGCTGATTCTGCTGTCCTTTCTGGTGGTTTTGACCGTCATTGATATAAAAACGTTACTGCTGCCAGATGTGCTT
ACGCTTTCGCTCTTGTGGATGGGACTGCTGTTTAATCTGTCGGAGACCTTTGTCCCATTACGCGATGCCGTAGTAGGCGC
GATGGCGGGCTATTTGTCCCTGTGGCTACTTTATTGGGTATTTAAATATGCCACAGGCAAAGAAGCGCTAGGGTATGGTG
ATTTTAAGTTACTCGCCGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTCTTGGTGGCGGCGCTGAGC
GGGCTGGTCGTTACTCTCATCTGGCGCGGGGTGCGTAAAGAAGATACGGCTAAACCGTTGGCCTTTGGCCCCTGGCTGGC
GATTGGCGGCGTGTTCGGTGTGATAATAAACGGACTCTATCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.37	96.085	0.484
pilD	Neisseria gonorrhoeae MS11	48.617	90.036	0.438
pilD	Vibrio campbellii strain DS40M4	46.85	90.391	0.423
pilD	Acinetobacter baumannii D1279779	41.971	97.509	0.409
pilD	Acinetobacter nosocomialis M2	41.971	97.509	0.409