Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   KUH140013_RS02475 Genome accession   NZ_AP020324
Coordinates   453066..453563 (+) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain KUN1163     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448066..458563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUH140013_RS02435 (KUN1163_0448) - 448110..449096 (+) 987 WP_000161311.1 ROK family glucokinase -
  KUH140013_RS02440 (KUN1163_0449) - 449096..449425 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KUH140013_RS02445 (KUN1163_0450) - 449422..450045 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KUH140013_RS02450 (KUN1163_0451) comGA 450097..451071 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KUH140013_RS02455 (KUN1163_0452) comGB 451043..452113 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  KUH140013_RS02460 (KUN1163_0453) comGC 452127..452438 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  KUH140013_RS02465 (KUN1163_0454) comGD 452416..452862 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  KUH140013_RS02470 (KUN1163_0455) comGE 452849..453148 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  KUH140013_RS02475 (KUN1163_0456) comGF 453066..453563 (+) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  KUH140013_RS02480 - 453660..453806 (+) 147 WP_001792109.1 hypothetical protein -
  KUH140013_RS02485 (KUN1163_0457) - 453796..454320 (+) 525 WP_001015120.1 shikimate kinase -
  KUH140013_RS02490 (KUN1163_0458) gcvT 454479..455570 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  KUH140013_RS02495 (KUN1163_0459) gcvPA 455590..456936 (+) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  KUH140013_RS02500 (KUN1163_0460) gcvPB 456929..458401 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=74844 KUH140013_RS02475 WP_001788897.1 453066..453563(+) (comGF) [Staphylococcus aureus strain KUN1163]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=74844 KUH140013_RS02475 WP_001788897.1 453066..453563(+) (comGF) [Staphylococcus aureus strain KUN1163]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment