Detailed information NTG0748421

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: KP2022_RS20730
• Genome accession: NZ_CP109604
• Coordinates: 4237122..4237922 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain KP2022
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4232122..4242922

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KP2022_RS20695 (KP2022_20700)	gspH	4232278..4232787 (+)	510	WP_064153121.1	type II secretion system minor pseudopilin GspH	-
KP2022_RS20700 (KP2022_20705)	gspI	4232790..4233155 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
KP2022_RS20705 (KP2022_20710)	gspJ	4233152..4233748 (+)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
KP2022_RS20710 (KP2022_20715)	gspK	4233745..4234731 (+)	987	WP_004177385.1	type II secretion system minor pseudopilin GspK	-
KP2022_RS20715 (KP2022_20720)	gspL	4234718..4235911 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
KP2022_RS20720 (KP2022_20725)	-	4235904..4236389 (+)	486	WP_004151943.1	type II secretion system protein M	-
KP2022_RS20725 (KP2022_20730)	-	4236379..4237125 (+)	747	WP_004145897.1	type II secretion system protein N	-
KP2022_RS20730 (KP2022_20735)	pilD	4237122..4237922 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
KP2022_RS20735 (KP2022_20740)	hrpB	4237970..4240399 (-)	2430	WP_032437605.1	ATP-dependent helicase HrpB	-
KP2022_RS20740 (KP2022_20745)	thpR	4240472..4241008 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
KP2022_RS20745 (KP2022_20750)	sfsA	4241008..4241724 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
KP2022_RS20750 (KP2022_20755)	dksA	4241887..4242342 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=748421 KP2022_RS20730 WP_004145898.1 4237122..4237922(+) (pilD) [Klebsiella pneumoniae strain KP2022]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=748421 KP2022_RS20730 WP_004145898.1 4237122..4237922(+) (pilD) [Klebsiella pneumoniae strain KP2022]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425