Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   TthHC11_RS06575 Genome accession   NZ_AP019801
Coordinates   1221490..1222191 (+) Length   233 a.a.
NCBI ID   WP_024119434.1    Uniprot ID   -
Organism   Thermus thermophilus strain HC11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1216490..1227191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TthHC11_RS06545 (TthHC11_12760) ilvB 1216936..1218624 (-) 1689 WP_024119431.1 biosynthetic-type acetolactate synthase large subunit -
  TthHC11_RS06555 (TthHC11_12770) - 1218779..1219759 (-) 981 WP_024119432.1 YpdA family putative bacillithiol disulfide reductase -
  TthHC11_RS06560 (TthHC11_12780) pilA/pilA1 1219917..1220387 (+) 471 WP_011173283.1 GspH/FimT family protein Machinery gene
  TthHC11_RS06565 (TthHC11_12790) - 1220442..1220915 (+) 474 WP_143591260.1 GspH/FimT family protein -
  TthHC11_RS06570 (TthHC11_12800) pilA/pilA2 1220933..1221493 (+) 561 WP_242003979.1 type II secretion system protein Machinery gene
  TthHC11_RS06575 (TthHC11_12810) pilA/pilA3 1221490..1222191 (+) 702 WP_024119434.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  TthHC11_RS06580 (TthHC11_12820) comZ 1222202..1223866 (+) 1665 WP_143591261.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  TthHC11_RS12010 (TthHC11_12830) pilA/pilA4 1224096..1224491 (+) 396 WP_011173287.1 type IV wide pilus major component PilA4 Machinery gene
  TthHC11_RS06590 - 1224548..1225840 (-) 1293 WP_143591263.1 hypothetical protein -
  TthHC11_RS06595 (TthHC11_12850) - 1226097..1226609 (+) 513 WP_011173288.1 hypothetical protein -

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 24979.48 Da        Isoelectric Point: 7.9063

>NTDB_id=74169 TthHC11_RS06575 WP_024119434.1 1221490..1222191(+) (pilA/pilA3) [Thermus thermophilus strain HC11]
MKRGFTLVEVLVAMAILVVVLAVGVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGSQNRAFSLPL
PPLSGSDMGPKDTLTLYYVTSLRDLASACRRVDYGFEGDTLRRSDVNATPSSGSDCTTPPPNSQPLAEGMLALDIQYQCS
DGSRKDTPDCGTDAYPRSAKVTVAGYSLTSVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide


Download         Length: 702 bp        

>NTDB_id=74169 TthHC11_RS06575 WP_024119434.1 1221490..1222191(+) (pilA/pilA3) [Thermus thermophilus strain HC11]
ATGAAGCGCGGGTTCACCCTTGTGGAGGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGGCGTGCGCTA
CTTCGCAAGCACCAGCGAGCTCGCCCGGAACACCCAGGCAAGGAGCGAACTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGACCTGCAGATGGCGGGGGCCCGCTACTGGAATAGCGGCAGCCAGAACCGGGCCTTCAGCCTCCCCCTT
CCCCCGCTTTCGGGAAGCGACATGGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGAGGGGGACACCCTACGCCGGAGCGACGTGAACGCCACCCCCTCTTCGGGAA
GCGATTGCACCACCCCCCCTCCGAACTCGCAGCCCTTGGCCGAAGGGATGCTGGCCCTGGACATCCAATACCAGTGCAGC
GACGGAAGCCGGAAGGACACGCCCGACTGCGGTACGGACGCCTACCCCCGCTCGGCCAAGGTCACGGTGGCAGGCTACTC
CCTGACCTCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCCGGG
CCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

98.712

100

0.987

Multiple sequence alignment