Detailed information NTG0074156

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: TthHC11_RS00090
• Genome accession: NZ_AP019801
• Coordinates: 18845..21514 (-)
• Length: 889 a.a.
• NCBI ID: WP_143590146.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain HC11
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 13845..26514

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TthHC11_RS00070 (TthHC11_00120)	-	14691..15323 (-)	633	WP_008634004.1	histidine phosphatase family protein	-
TthHC11_RS00075 (TthHC11_00130)	purM	15320..16321 (-)	1002	WP_143590140.1	phosphoribosylformylglycinamidine cyclo-ligase	-
TthHC11_RS00080 (TthHC11_00140)	gatB	16327..17736 (-)	1410	WP_143590142.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
TthHC11_RS00085 (TthHC11_00150)	pilT	17747..18832 (-)	1086	WP_143590144.1	type IV pilus twitching motility protein PilT	Machinery gene
TthHC11_RS00090 (TthHC11_00160)	pilF	18845..21514 (-)	2670	WP_143590146.1	type IV pilus assembly ATPase PilB	Machinery gene
TthHC11_RS00095 (TthHC11_00170)	-	21511..21996 (-)	486	WP_011173992.1	YqeG family HAD IIIA-type phosphatase	-
TthHC11_RS00100 (TthHC11_00180)	pgeF	22031..22759 (-)	729	WP_143590148.1	peptidoglycan editing factor PgeF	-
TthHC11_RS00105 (TthHC11_00190)	surE	22756..23511 (-)	756	WP_011173994.1	5'/3'-nucleotidase SurE	-
TthHC11_RS00110 (TthHC11_00200)	-	23570..23776 (-)	207	WP_011173995.1	cold-shock protein	-
TthHC11_RS00115 (TthHC11_00210)	-	23933..25222 (+)	1290	WP_143590150.1	ABC transporter substrate-binding protein	-
TthHC11_RS00120 (TthHC11_00220)	-	25274..26149 (+)	876	WP_011173997.1	sugar ABC transporter permease	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 98257.39 Da
• Isoelectric Point: 5.3348

>NTDB_id=74156 TthHC11_RS00090 WP_143590146.1 18845..21514(-) (pilF) [Thermus thermophilus strain HC11]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSAFLYALAKHYPELGLPVPPPPSG
EGQKDLKLGELLLQKGWISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESTEGIVPDPSAALLL
LRSDALRYGAVPIGFQNGEVEVVLSDPRHKEAVAQLLNRPARFYLTLPQAWEELFRRAYPQKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRRGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYIDPEEDPP
DPGAPLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNYEVAPAVATEAAITKLIERFYGKAEL
SEIAKEFAKKQAEEEVPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVI
SVVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGL
SEAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTL
EEVLARTIE

Nucleotide

• Length: 2670 bp

>NTDB_id=74156 TthHC11_RS00090 WP_143590146.1 18845..21514(-) (pilF) [Thermus thermophilus strain HC11]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTCAAG
GCCCTCCTTCCCGCGGAGAAGGCCAAGGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTGGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAGA
CCACCAAAAGCGCCTTCCTCTACGCCCTGGCCAAACACTACCCGGAGCTCGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAGAAGGACCTCAAGCTCGGGGAGCTCCTCCTGCAAAAGGGGTGGATCTCCCGCGAGGCCCTGGAGGAGGCCTT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CCTTGGCGGAGCAGAAGGGGCTGGAGTTTCTGGAAAGCACCGAGGGGATCGTCCCAGACCCCTCCGCCGCCCTCCTCCTC
CTCCGCTCCGACGCCCTGCGTTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGGAGGTGGAGGTGGTCCTCTCCGACCC
CCGGCACAAGGAGGCGGTGGCCCAGCTCCTCAATCGGCCCGCCCGCTTCTACCTCACCCTCCCCCAGGCCTGGGAGGAGC
TCTTCCGCCGGGCCTACCCGCAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAAGCGCTG
AAGGAGGCCCTGGAGGTGCAGAAAGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCGGGGCGGGGGCCGGCTGGAGGACACCCTGGTCCAGTCGGGGA
AGCTCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTATATTGACCCCGAGGAGGACCCCCCC
GATCCCGGCGCCCCCCTCCTCCTCCCAGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAAACCG
CCTCGTCCTCCTGATGAAGGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGAGGAAGGGCCTGA
ACTACGAAGTGGCCCCCGCCGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAGTTCGCCAAAAAGCAGGCGGAGGAGGAGGTCCCAAGCCCCCTGGAGCTGGACGAGAGCGCCGC
CCAGAAGTTCGTGAAGCAGGTGATCCGGGAAGCCTTCCTCCAGGACGCCTCCGACATCCACATTGAGCCCAGGCAGAACG
ACGTCCAGGTGCGCCTCCGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCCAAGGGGGCGCTGAACGCGGTGATC
TCCGTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAGCGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGG
GGCCATAGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGG
CCTCGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGGCCCACGGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATTGAAGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCCC
AGGCGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGG
GACTCCGAGACGGCCAAGATCGCCACCGAAGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCCCTTCAACATCTCCGCGGCCCTCATCGGCGTCCTCTCCC
AGCGCCTGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTT
TCCGAGGCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGTGAGCGGTGCGGCGGCACCGGGTACAAGGGCCG
CTACGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCA
AGGAGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

• Type II/IV secretion system protein (T2SSE) (513-779)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	99.775	100	0.998
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.341	100	0.565