Detailed information NTG0740306

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH531_RS08720
• Genome accession: NZ_CP107498
• Coordinates: 1741009..1741809 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1736009..1746809

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH531_RS08685	gspH	1736165..1736680 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
OH531_RS08690	gspI	1736677..1737042 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH531_RS08695	gspJ	1737039..1737635 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
OH531_RS08700	gspK	1737632..1738618 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
OH531_RS08705	gspL	1738605..1739798 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
OH531_RS08710	-	1739791..1740276 (+)	486	WP_004151943.1	type II secretion system protein M	-
OH531_RS08715	-	1740266..1741012 (+)	747	WP_004145897.1	type II secretion system protein N	-
OH531_RS08720	pilD	1741009..1741809 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH531_RS08725	hrpB	1741857..1744286 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
OH531_RS08730	thpR	1744359..1744895 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH531_RS08735	sfsA	1744895..1745611 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH531_RS08740	dksA	1745774..1746229 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=740306 OH531_RS08720 WP_002888947.1 1741009..1741809(+) (pilD) [Klebsiella pneumoniae strain CRKP_5]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=740306 OH531_RS08720 WP_002888947.1 1741009..1741809(+) (pilD) [Klebsiella pneumoniae strain CRKP_5]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425