Detailed information NTG0740186

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH522_RS08865
• Genome accession: NZ_CP107473
• Coordinates: 1771071..1771871 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1766071..1776871

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH522_RS08830	gspH	1766227..1766742 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
OH522_RS08835	gspI	1766739..1767104 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH522_RS08840	gspJ	1767101..1767697 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
OH522_RS08845	gspK	1767694..1768680 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
OH522_RS08850	gspL	1768667..1769860 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
OH522_RS08855	-	1769853..1770338 (+)	486	WP_004151943.1	type II secretion system protein M	-
OH522_RS08860	-	1770328..1771074 (+)	747	WP_004145897.1	type II secretion system protein N	-
OH522_RS08865	pilD	1771071..1771871 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH522_RS08870	hrpB	1771919..1774348 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
OH522_RS08875	thpR	1774421..1774957 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH522_RS08880	sfsA	1774957..1775673 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH522_RS08885	dksA	1775836..1776291 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=740186 OH522_RS08865 WP_002888947.1 1771071..1771871(+) (pilD) [Klebsiella pneumoniae strain CRKP_10]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=740186 OH522_RS08865 WP_002888947.1 1771071..1771871(+) (pilD) [Klebsiella pneumoniae strain CRKP_10]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425