Detailed information NTG0739946

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH530_RS08805
• Genome accession: NZ_CP107422
• Coordinates: 1757403..1758203 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1752403..1763203

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH530_RS08770	gspH	1752559..1753074 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
OH530_RS08775	gspI	1753071..1753436 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH530_RS08780	gspJ	1753433..1754029 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
OH530_RS08785	gspK	1754026..1755012 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
OH530_RS08790	gspL	1754999..1756192 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
OH530_RS08795	-	1756185..1756670 (+)	486	WP_004151943.1	type II secretion system protein M	-
OH530_RS08800	-	1756660..1757406 (+)	747	WP_004145897.1	type II secretion system protein N	-
OH530_RS08805	pilD	1757403..1758203 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH530_RS08810	hrpB	1758251..1760680 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
OH530_RS08815	thpR	1760753..1761289 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH530_RS08820	sfsA	1761289..1762005 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH530_RS08825	dksA	1762168..1762623 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=739946 OH530_RS08805 WP_002888947.1 1757403..1758203(+) (pilD) [Klebsiella pneumoniae strain CRKP_20]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=739946 OH530_RS08805 WP_002888947.1 1757403..1758203(+) (pilD) [Klebsiella pneumoniae strain CRKP_20]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425