Detailed information NTG0739888

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH533_RS22705
• Genome accession: NZ_CP107408
• Coordinates: 4545299..4546099 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_24
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4540299..4551099

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH533_RS22670	gspH	4540455..4540970 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
OH533_RS22675	gspI	4540967..4541332 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH533_RS22680	gspJ	4541329..4541925 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
OH533_RS22685	gspK	4541922..4542908 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
OH533_RS22690	gspL	4542895..4544088 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
OH533_RS22695	-	4544081..4544566 (+)	486	WP_004151943.1	type II secretion system protein M	-
OH533_RS22700	-	4544556..4545302 (+)	747	WP_004145897.1	type II secretion system protein N	-
OH533_RS22705	pilD	4545299..4546099 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH533_RS22710	hrpB	4546147..4548576 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
OH533_RS22715	thpR	4548649..4549185 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH533_RS22720	sfsA	4549185..4549901 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH533_RS22725	dksA	4550064..4550519 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=739888 OH533_RS22705 WP_002888947.1 4545299..4546099(+) (pilD) [Klebsiella pneumoniae strain CRKP_24]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=739888 OH533_RS22705 WP_002888947.1 4545299..4546099(+) (pilD) [Klebsiella pneumoniae strain CRKP_24]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425