Detailed information NTG0739610

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH515_RS08120
• Genome accession: NZ_CP107352
• Coordinates: 1667686..1668486 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_35
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1662686..1673486

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH515_RS08085	gspH	1662842..1663357 (+)	516	WP_004213437.1	type II secretion system minor pseudopilin GspH	-
OH515_RS08090	gspI	1663354..1663719 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH515_RS08095	gspJ	1663716..1664312 (+)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
OH515_RS08100	gspK	1664309..1665295 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
OH515_RS08105	gspL	1665282..1666475 (+)	1194	WP_019704440.1	type II secretion system protein GspL	-
OH515_RS08110	-	1666468..1666953 (+)	486	WP_023312675.1	type II secretion system protein M	-
OH515_RS08115	-	1666943..1667689 (+)	747	WP_004145897.1	type II secretion system protein N	-
OH515_RS08120	pilD	1667686..1668486 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH515_RS08125	hrpB	1668534..1670963 (-)	2430	WP_065808762.1	ATP-dependent helicase HrpB	-
OH515_RS08130	thpR	1671036..1671572 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH515_RS08135	sfsA	1671572..1672288 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH515_RS08140	dksA	1672451..1672906 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=739610 OH515_RS08120 WP_002888947.1 1667686..1668486(+) (pilD) [Klebsiella pneumoniae strain CRKP_35]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=739610 OH515_RS08120 WP_002888947.1 1667686..1668486(+) (pilD) [Klebsiella pneumoniae strain CRKP_35]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425