Detailed information NTG0739600

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH497_RS22425
• Genome accession: NZ_CP107345
• Coordinates: 4497720..4498520 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_36
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4492720..4503520

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH497_RS22390	gspH	4492876..4493391 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
OH497_RS22395	gspI	4493388..4493753 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH497_RS22400	gspJ	4493750..4494346 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
OH497_RS22405	gspK	4494343..4495329 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
OH497_RS22410	gspL	4495316..4496509 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
OH497_RS22415	-	4496502..4496987 (+)	486	WP_004151943.1	type II secretion system protein M	-
OH497_RS22420	-	4496977..4497723 (+)	747	WP_004145897.1	type II secretion system protein N	-
OH497_RS22425	pilD	4497720..4498520 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH497_RS22430	hrpB	4498568..4500997 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
OH497_RS22435	thpR	4501070..4501606 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH497_RS22440	sfsA	4501606..4502322 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH497_RS22445	dksA	4502485..4502940 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=739600 OH497_RS22425 WP_002888947.1 4497720..4498520(+) (pilD) [Klebsiella pneumoniae strain CRKP_36]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=739600 OH497_RS22425 WP_002888947.1 4497720..4498520(+) (pilD) [Klebsiella pneumoniae strain CRKP_36]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425