Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OFO16_RS13700 Genome accession   NZ_CP107282
Coordinates   2962804..2963820 (-) Length   338 a.a.
NCBI ID   WP_263837579.1    Uniprot ID   -
Organism   Vibrio natriegens strain PWH3a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2957804..2968820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OFO16_RS13675 (OFO16_13675) aroK 2958623..2959141 (-) 519 WP_014233147.1 shikimate kinase AroK -
  OFO16_RS13680 (OFO16_13680) pilQ 2959330..2961069 (-) 1740 WP_263837575.1 type IV pilus secretin PilQ Machinery gene
  OFO16_RS13685 (OFO16_13685) pilP 2961154..2961669 (-) 516 WP_263837576.1 pilus assembly protein PilP Machinery gene
  OFO16_RS13690 (OFO16_13690) pilO 2961659..2962252 (-) 594 WP_263837577.1 type 4a pilus biogenesis protein PilO Machinery gene
  OFO16_RS13695 (OFO16_13695) pilN 2962245..2962820 (-) 576 WP_263837578.1 PilN domain-containing protein Machinery gene
  OFO16_RS13700 (OFO16_13700) pilM 2962804..2963820 (-) 1017 WP_263837579.1 type IV pilus assembly protein PilM Machinery gene
  OFO16_RS13705 (OFO16_13705) - 2963964..2966531 (+) 2568 WP_263837580.1 penicillin-binding protein 1A -
  OFO16_RS13710 (OFO16_13710) oxyR 2966599..2967507 (-) 909 WP_263837581.1 DNA-binding transcriptional regulator OxyR -
  OFO16_RS13715 (OFO16_13715) - 2967666..2968394 (+) 729 WP_263837582.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37665.00 Da        Isoelectric Point: 6.9712

>NTDB_id=739286 OFO16_RS13700 WP_263837579.1 2962804..2963820(-) (pilM) [Vibrio natriegens strain PWH3a]
MDKRIVTGIDIGHNSLKAVVLKPNGEQFALLGYKEILLKDGIVAENNTINHQEIVNTLKQVKKDLPFGAIRVAISVPDNA
VISKKLHIEQDLEENEIEFAVVQAFSHQSPFPVDELSLDFVRLNHEGGLRGPVSYQVFATRKDVVESRIEAIRKSGFKPV
LVEVHSQSLGQIWKLATERFPDKSTYGLLDVGSVSSSFTMFTEQNELFHKEFACGTGSIQCSVQESLLPGEEQAKTNQFN
RQMVDRVKRQIQLYTSINGSQNIKGIWLSGEGATTPMLAEELSHQLAIECELLNPLGLFEMNVAKRKRRAADWQHFSTAA
GLAVRGIQWLGGRRALSN

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=739286 OFO16_RS13700 WP_263837579.1 2962804..2963820(-) (pilM) [Vibrio natriegens strain PWH3a]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGAGCA
ATTCGCCTTGTTGGGTTACAAAGAAATATTACTTAAGGATGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGGTGAAAAAAGACCTGCCATTTGGTGCAATCAGAGTGGCAATTTCAGTGCCTGATAATGCG
GTTATCAGCAAAAAACTGCACATCGAACAAGACCTTGAAGAGAACGAAATAGAGTTCGCGGTCGTGCAGGCATTTTCTCA
TCAATCTCCTTTCCCCGTAGATGAGTTGAGTCTGGACTTTGTCCGTCTTAATCATGAAGGAGGCCTGCGAGGGCCTGTTA
GCTACCAAGTTTTTGCGACACGTAAAGATGTCGTGGAGAGTCGGATTGAAGCAATACGTAAATCCGGTTTTAAACCTGTC
CTTGTCGAGGTCCATTCACAAAGCCTCGGGCAGATATGGAAACTGGCCACAGAACGTTTTCCAGATAAAAGTACTTACGG
TTTGCTTGATGTTGGTTCTGTGTCGAGCTCTTTCACGATGTTCACCGAGCAAAACGAGCTATTTCATAAAGAATTTGCCT
GTGGAACAGGTTCCATTCAGTGCTCTGTGCAGGAAAGTCTTCTGCCCGGAGAAGAACAAGCCAAAACAAATCAATTCAAT
CGTCAGATGGTCGATCGCGTCAAACGTCAGATACAGCTTTATACGTCGATTAACGGGTCACAAAACATCAAAGGTATTTG
GTTGTCTGGTGAAGGTGCAACAACGCCAATGCTGGCGGAGGAACTATCTCATCAATTGGCGATAGAGTGTGAGTTACTGA
ACCCGTTAGGCTTATTTGAAATGAACGTCGCTAAACGTAAGCGTCGAGCTGCAGACTGGCAGCACTTTTCAACCGCTGCT
GGCCTGGCAGTACGAGGTATCCAATGGTTAGGAGGTCGCCGTGCTCTATCGAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

75.148

100

0.751
  pilM Vibrio cholerae strain A1552

49.254

99.112

0.488