Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ODS73_RS11850 Genome accession   NZ_CP107038
Coordinates   2261561..2262859 (-) Length   432 a.a.
NCBI ID   WP_050095242.1    Uniprot ID   A0A4J2AYZ8
Organism   Streptococcus pneumoniae strain BM6001     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2256561..2267859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ODS73_RS11830 (ODS73_11820) rplI 2257716..2258168 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  ODS73_RS11835 (ODS73_11825) - 2258165..2260138 (-) 1974 WP_000715111.1 DHH family phosphoesterase -
  ODS73_RS11840 (ODS73_11830) hpf 2260274..2260822 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ODS73_RS11845 (ODS73_11835) comFC/cflB 2260902..2261564 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  ODS73_RS11850 (ODS73_11840) comFA/cflA 2261561..2262859 (-) 1299 WP_050095242.1 DEAD/DEAH box helicase Machinery gene
  ODS73_RS11855 (ODS73_11845) - 2262915..2263550 (+) 636 WP_000395730.1 YigZ family protein -
  ODS73_RS11860 (ODS73_11850) cysK 2263835..2264755 (+) 921 WP_000029866.1 cysteine synthase A -
  ODS73_RS11865 (ODS73_11855) - 2264830..2265368 (-) 539 Protein_2314 transposase -
  ODS73_RS11870 (ODS73_11860) - 2265341..2265628 (+) 288 Protein_2315 transposase -
  ODS73_RS11875 (ODS73_11865) tnpB 2265819..2266106 (-) 288 WP_000586649.1 IS66 family insertion sequence element accessory protein TnpB -
  ODS73_RS11880 (ODS73_11870) tsf 2266352..2267392 (-) 1041 WP_050220156.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49590.61 Da        Isoelectric Point: 8.7540

>NTDB_id=737599 ODS73_RS11850 WP_050095242.1 2261561..2262859(-) (comFA/cflA) [Streptococcus pneumoniae strain BM6001]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKRKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=737599 ODS73_RS11850 WP_050095242.1 2261561..2262859(-) (comFA/cflA) [Streptococcus pneumoniae strain BM6001]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGATAAGCGAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGTATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4J2AYZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae D39

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae R6

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae TIGR4

99.537

100

0.995
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA/cflA Streptococcus mitis SK321

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

52.451

94.444

0.495
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387