Detailed information NTG0736866

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OCU08_RS21925
• Genome accession: NZ_CP106964
• Coordinates: 4515730..4516530 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain SY1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4510730..4521530

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCU08_RS21890	gspH	4510883..4511398 (+)	516	WP_046882113.1	type II secretion system minor pseudopilin GspH	-
OCU08_RS21895	gspI	4511395..4511760 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
OCU08_RS21900	gspJ	4511757..4512353 (+)	597	WP_072121920.1	type II secretion system minor pseudopilin GspJ	-
OCU08_RS21905	gspK	4512350..4513330 (+)	981	WP_077263600.1	type II secretion system minor pseudopilin GspK	-
OCU08_RS21910	gspL	4513323..4514519 (+)	1197	WP_046620843.1	type II secretion system protein GspL	-
OCU08_RS21915	-	4514512..4514997 (+)	486	WP_012968921.1	type II secretion system protein M	-
OCU08_RS21920	-	4514987..4515733 (+)	747	WP_049157077.1	type II secretion system protein N	-
OCU08_RS21925	pilD	4515730..4516530 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
OCU08_RS21930	hrpB	4516577..4519006 (-)	2430	WP_101880591.1	ATP-dependent helicase HrpB	-
OCU08_RS21935	thpR	4519079..4519615 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
OCU08_RS21940	sfsA	4519615..4520331 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
OCU08_RS21945	dksA	4520494..4520949 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=736866 OCU08_RS21925 WP_008807384.1 4515730..4516530(+) (pilD) [Klebsiella variicola strain SY1]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=736866 OCU08_RS21925 WP_008807384.1 4515730..4516530(+) (pilD) [Klebsiella variicola strain SY1]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425