Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N8Z00_RS02990 Genome accession   NZ_CP106885
Coordinates   674203..675648 (-) Length   481 a.a.
NCBI ID   WP_268080308.1    Uniprot ID   -
Organism   Achromobacter spanius strain LIG3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 669203..680648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8Z00_RS02965 (N8Z00_02965) - 669723..670352 (+) 630 WP_268080305.1 LysE family translocator -
  N8Z00_RS02970 (N8Z00_02970) - 670466..672073 (+) 1608 WP_268080306.1 peptide chain release factor 3 -
  N8Z00_RS02975 (N8Z00_02975) - 672098..672784 (+) 687 WP_268080307.1 hypothetical protein -
  N8Z00_RS02980 (N8Z00_02980) bfr 672859..673335 (-) 477 WP_050448876.1 bacterioferritin -
  N8Z00_RS02985 (N8Z00_02985) - 673800..674081 (-) 282 WP_050448875.1 (2Fe-2S)-binding protein -
  N8Z00_RS02990 (N8Z00_02990) rpoN/rpoN1 674203..675648 (-) 1446 WP_268080308.1 RNA polymerase factor sigma-54 Machinery gene
  N8Z00_RS02995 (N8Z00_02995) - 675647..676279 (+) 633 WP_268080309.1 hypothetical protein -
  N8Z00_RS03000 (N8Z00_03000) leuA 676639..678351 (+) 1713 WP_268080310.1 2-isopropylmalate synthase -
  N8Z00_RS03005 (N8Z00_03005) - 678507..679835 (-) 1329 WP_268080311.1 amidase -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52568.90 Da        Isoelectric Point: 4.8503

>NTDB_id=736438 N8Z00_RS02990 WP_268080308.1 674203..675648(-) (rpoN/rpoN1) [Achromobacter spanius strain LIG3]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLADNPLLEREDDTPAAEAQAESERESSVQDDEP
AVERETPVDEMPGSGGVYSDDDSGLPEAARPDTLREHLLGQLMLTRAAPRDAALAALLIDELDENGYLGSPLEEILTWLP
ADMEPDLDELRAALSLLQSFDPPGIGARDMADCLVLQLRHPDLSRLPEAADGAVLACARVICAQHLPVLATGNAARLCAA
VGCDEATFRAAHSLILRLEPRPGRAWTVPAADYAVPDVIVRKTRRGWQVTLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QTQLQQARWMIRNVEQRFDTILRVAQAIVERQTAFFSQGPSAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTDGGDATSATAVQTFIRQMVAEENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPATLRKAQASA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=736438 N8Z00_RS02990 WP_268080308.1 674203..675648(-) (rpoN/rpoN1) [Achromobacter spanius strain LIG3]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGACCTGGCCAGCATTTGACGCTGACCCCTCAACTGCA
GCAATCGATACGATTGTTGCAGTTGTCCACGCTCGACCTTGAGGCCGAGATCTCGCAAGTGCTGGCGGACAACCCGCTGC
TGGAGCGTGAAGACGACACCCCCGCCGCCGAGGCGCAAGCCGAATCCGAGCGCGAATCCTCCGTGCAGGACGATGAGCCC
GCGGTAGAGCGCGAGACGCCAGTGGACGAAATGCCCGGCTCGGGCGGGGTCTATTCGGATGATGATTCCGGCCTGCCCGA
GGCGGCGCGCCCCGACACCCTGCGCGAGCATCTGCTGGGCCAATTGATGTTGACTCGGGCCGCCCCGCGTGACGCCGCGC
TGGCTGCGCTGCTGATCGACGAACTGGACGAAAACGGGTATCTGGGGTCGCCGCTAGAGGAAATCCTGACGTGGCTGCCC
GCCGACATGGAGCCGGACCTGGACGAATTGCGCGCTGCGCTGTCCTTGTTGCAGTCGTTCGACCCGCCTGGCATCGGTGC
GCGAGACATGGCCGACTGCCTGGTGTTGCAATTGCGCCATCCCGACCTGTCCCGGTTGCCGGAAGCGGCTGACGGGGCGG
TTTTAGCGTGCGCCCGGGTTATCTGCGCGCAGCATCTGCCCGTACTGGCCACGGGTAACGCCGCTCGCCTTTGCGCCGCC
GTGGGCTGTGACGAGGCGACCTTTCGTGCTGCCCATTCGCTGATCCTGCGGTTGGAACCTCGCCCCGGACGGGCCTGGAC
GGTGCCGGCGGCTGATTACGCCGTGCCGGACGTCATCGTGCGCAAGACCCGGCGCGGGTGGCAAGTCACGCTCAACAGCG
CTGCCGTGCCCCGGTTGCAGATCAACGGCCTGTATGCGCAGATGCTGGGCAACCAGAAGGAATCCGCGCACGCGGGCCTG
CAGACCCAATTGCAGCAAGCCAGATGGATGATCCGCAACGTGGAGCAGCGCTTTGACACGATCCTGCGGGTCGCCCAGGC
GATCGTGGAACGCCAGACCGCGTTCTTCAGCCAGGGGCCGTCAGCTATGCGCCCGTTGATCCTGAAAGACATTGCGGGCG
AGCTCGGATTGCACGAATCGACCATTTCACGGGCTACAACACAGAAGTACATGCTCACTCCGTTCGGCACGCTGGAGCTC
AAGCGCTTCTTCGGCACGGGCGTCTCCACGGACGGCGGCGATGCAACGTCCGCCACGGCGGTGCAGACGTTCATCCGCCA
AATGGTGGCTGAAGAGAACCGTGCCAAGCCCTTGTCCGATAGCCAGATCATGCAGAAACTGGCCGATCAGGGGATAGTCA
TCGCCCGTCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGGATTGCCCCCGCCACGCTGCGCAAGGCACAGGCTAGCGCG
CGTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.713

100

0.511