Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   DO80_RS00105 Genome accession   NZ_AP019730
Coordinates   14324..16687 (+) Length   787 a.a.
NCBI ID   WP_108304348.1    Uniprot ID   -
Organism   Helicobacter pylori strain TN2wt     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 9324..21687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DO80_RS00070 (DO80_00090) groES 9355..9711 (-) 357 WP_000671954.1 co-chaperone GroES -
  DO80_RS00080 (DO80_00100) dnaG 9987..11666 (+) 1680 WP_108304346.1 DNA primase -
  DO80_RS00085 (DO80_00110) - 11663..12715 (+) 1053 WP_024115846.1 MnmA/TRMU family protein -
  DO80_RS00090 (DO80_00120) - 12806..13633 (+) 828 WP_108304347.1 DUF5718 family protein -
  DO80_RS00095 (DO80_00130) comB2 13778..14059 (+) 282 WP_001272686.1 TrbC/VirB2 family protein Machinery gene
  DO80_RS00100 (DO80_00140) comB3 14059..14322 (+) 264 WP_000584955.1 hypothetical protein Machinery gene
  DO80_RS00105 (DO80_00150) comB4 14324..16687 (+) 2364 WP_108304348.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  DO80_RS00115 (DO80_00160) - 17007..18347 (+) 1341 WP_172620318.1 COG3014 family protein -
  DO80_RS00120 (DO80_00170) cheV1 18448..19413 (+) 966 WP_000785465.1 chemotaxis protein CheV1 -
  DO80_RS00125 (DO80_00180) nspC 19410..20627 (+) 1218 WP_108304350.1 carboxynorspermidine decarboxylase -
  DO80_RS00130 (DO80_00190) lpxE 20637..21170 (-) 534 WP_232051529.1 lipid A 1-phosphatase LpxE -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 90290.01 Da        Isoelectric Point: 8.9895

>NTDB_id=73622 DO80_RS00105 WP_108304348.1 14324..16687(+) (comB4) [Helicobacter pylori strain TN2wt]
MLEKLLSAIKQKVSNYFLGVLPKSYSMSEENNILGLYDEHFLLTKNENLVGILRLEGVSYTHLSTEQLQDLFTERQMALD
SLEKVVVRLVVKRRKIDHQQNIQSDSKYLQAILNQFENKEVYENQYFLVLESAHSLQGVLEHKKKSLMHANRENFKDILS
YKAYFLQETLKSLEIQLKNYAPKLLSSKEVLNFYAEYINGFDLPLKPLVGGYLSDSYIASSITFEKDYFIQESFNQKTYN
RLIGIKAYESERITSIAIGALLYQETPLDIIFSIEPMSVNKTLSFLKERAKFSMSNLVKNELLEYQELVKTKRLSMQKFA
LNILIKAPSLEDLDTQTSLVLGLLFKENLVGVIETFGLKGGYFSFFPERIHLNHRLRFLTSKALACLMVFERQNLGFKAN
SWGNSPLSVFKNLDYSPFLFNFHNQEVSHNNAKEIARVNGHTLIIGATGSGKSTLISFLMMSALKYQNMRLLAFDRMQGL
YSFTKFFKGHYHDGKSFSINPFCLEPNLQNLEFLQSFFLSMFDLAPSRDKEALEDMNAILGAIKSLYETLYPKAFSLLDF
KETLKRTSSNQLGLSLEPYLNNPLFNALNDAFNSSAFLNVINLDTITQNPKDLGLLAYYLFYKILEESRKNDSGFLVFLD
EFKSYVENDLLNAKINALITQARKANGVVVLALQDIYQLSGVKNAHSFLSNMGTLILYPQKNARELKHQFNMPLSETEIS
FLENTPLYARQVLVKNLGNGSSNMIDVSLESLGRYLKIFNSDSSHVNKVKALQKDYPKEWREKLLKS

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=73622 DO80_RS00105 WP_108304348.1 14324..16687(+) (comB4) [Helicobacter pylori strain TN2wt]
ATGTTAGAAAAGCTTTTGAGCGCCATCAAACAAAAAGTTTCAAACTATTTTTTAGGGGTTTTGCCTAAAAGCTATTCTAT
GAGCGAAGAAAACAACATTTTAGGCTTGTATGATGAGCATTTCTTGCTCACTAAAAACGAAAACTTAGTGGGAATCCTCC
GTTTAGAGGGGGTGAGTTACACCCATTTAAGCACGGAGCAATTGCAAGATCTCTTCACCGAGCGCCAAATGGCGTTGGAT
TCTTTAGAAAAAGTCGTGGTGCGCCTTGTGGTTAAAAGGCGTAAAATTGATCACCAACAAAACATTCAATCTGACTCTAA
ATACTTGCAAGCGATTTTGAATCAATTTGAAAATAAAGAAGTGTATGAGAATCAGTATTTTTTAGTTTTAGAAAGTGCGC
ATTCTTTACAAGGCGTTTTAGAACATAAGAAAAAATCCTTAATGCATGCCAATAGGGAAAATTTTAAGGACATTCTCTCT
TATAAAGCGTATTTTTTGCAAGAAACTTTAAAAAGCTTAGAAATCCAGCTCAAAAACTACGCCCCCAAACTCTTAAGCTC
TAAAGAGGTTTTGAATTTTTATGCGGAATACATTAACGGGTTTGATCTCCCTTTAAAGCCCCTAGTAGGGGGGTATTTGA
GCGACAGCTATATCGCTAGTTCTATCACTTTTGAAAAAGATTATTTCATTCAAGAAAGCTTTAATCAAAAAACCTATAAC
CGCTTGATTGGCATTAAAGCTTATGAGAGCGAGCGCATCACTTCTATAGCGATCGGAGCGCTTTTATACCAAGAAACGCC
CTTAGACATTATCTTTTCTATAGAGCCTATGAGCGTCAATAAAACGCTGAGTTTTTTAAAAGAGAGGGCCAAGTTTAGCA
TGTCCAATCTCGTTAAAAACGAGCTGCTAGAATACCAAGAACTGGTCAAAACCAAACGCCTATCCATGCAAAAATTCGCC
CTAAACATTCTTATCAAAGCCCCTAGTTTAGAGGATTTAGACACTCAAACAAGCTTAGTTTTAGGGCTTTTATTTAAAGA
AAACTTAGTGGGTGTTATAGAAACTTTTGGCTTGAAGGGGGGGTATTTTTCCTTTTTCCCTGAACGCATCCATTTAAACC
ACCGCTTGCGTTTTTTAACCTCTAAAGCCCTAGCGTGTTTAATGGTGTTTGAAAGGCAAAATTTAGGTTTTAAGGCTAAT
TCATGGGGGAATAGCCCTTTGAGCGTGTTTAAAAATTTGGATTATTCCCCCTTTTTATTCAATTTCCACAACCAAGAAGT
GAGCCACAACAACGCTAAAGAAATCGCCAGAGTGAATGGGCATACCTTAATTATAGGGGCTACCGGGAGCGGTAAAAGCA
CGCTGATTAGCTTTTTAATGATGAGCGCTTTGAAATACCAAAACATGCGCCTTTTAGCATTTGATAGGATGCAAGGGCTG
TATTCTTTCACAAAATTTTTTAAAGGGCATTACCATGACGGCAAATCTTTTAGCATCAACCCCTTTTGTTTAGAGCCTAA
TTTGCAGAATTTAGAATTTTTGCAATCCTTCTTTTTGAGCATGTTTGATCTTGCCCCTTCAAGGGATAAAGAAGCCTTAG
AAGACATGAATGCGATTTTGGGTGCGATTAAGAGCCTTTATGAGACTTTATACCCTAAAGCTTTTAGTTTGTTAGACTTT
AAAGAAACGCTTAAAAGAACCTCATCTAACCAATTGGGCTTGAGTTTAGAGCCGTATTTGAATAACCCTCTTTTTAACGC
CTTGAATGACGCGTTCAACTCTAGCGCTTTTTTAAATGTGATAAACCTAGACACCATCACCCAAAACCCTAAAGACTTAG
GGCTTTTAGCCTATTATTTGTTTTATAAAATCTTAGAAGAATCCAGGAAAAACGACAGCGGTTTTTTGGTTTTTTTAGAC
GAGTTTAAATCCTATGTGGAAAACGATTTGTTGAACGCTAAAATTAACGCTTTAATCACGCAAGCCAGAAAGGCTAATGG
TGTGGTGGTGTTGGCTTTGCAAGACATTTACCAATTAAGCGGGGTTAAAAACGCTCATAGTTTTTTAAGCAACATGGGGA
CTCTTATTTTGTACCCGCAAAAAAACGCTAGGGAGTTGAAACACCAATTCAATATGCCTTTGAGCGAAACTGAAATTTCT
TTTTTAGAAAACACCCCTTTGTATGCCAGGCAGGTTTTAGTCAAAAATCTGGGTAACGGGAGTTCTAACATGATTGATGT
GAGTTTGGAAAGTTTGGGGCGTTATTTGAAAATCTTTAATTCAGACTCTAGCCATGTGAATAAAGTGAAAGCGTTACAAA
AAGACTACCCTAAAGAGTGGCGTGAGAAACTTTTAAAGAGCTAG

Domains

Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-375)

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(426-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

97.586

100

0.976

Multiple sequence alignment