Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   P5670_RS19270 Genome accession   NZ_CP121087
Coordinates   3951132..3951941 (+) Length   269 a.a.
NCBI ID   WP_001605896.1    Uniprot ID   -
Organism   Escherichia coli strain ST3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3946132..3956941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5670_RS19265 (P5670_19265) sslE 3946446..3951002 (+) 4557 WP_001605897.1 lipoprotein metalloprotease SslE -
  P5670_RS19270 (P5670_19270) pilD 3951132..3951941 (+) 810 WP_001605896.1 prepilin peptidase PppA Machinery gene
  P5670_RS19275 (P5670_19275) gspS2 3952007..3952417 (+) 411 WP_001389459.1 type II secretion system pilot lipoprotein GspS-beta -
  P5670_RS19280 (P5670_19280) gspC 3952435..3953394 (+) 960 WP_024190281.1 type II secretion system protein GspC -
  P5670_RS19285 (P5670_19285) gspD 3953424..3955484 (+) 2061 WP_000498847.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29471.16 Da        Isoelectric Point: 8.3801

>NTDB_id=735574 P5670_RS19270 WP_001605896.1 3951132..3951941(+) (pilD) [Escherichia coli strain ST3]
MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
GLIAAWVQQSPLTLQDAVTGVLVGFIAFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=735574 P5670_RS19270 WP_001605896.1 3951132..3951941(+) (pilD) [Escherichia coli strain ST3]
ATGCTTTTTGATGTTTTTCAGCAATACCCTGCGGCGATGCCCATACTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCATGGTGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGCTCTCCTGGTTGATGCTCAAAGGGCGCTGCCGTGATTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACTGCACTCGCTTTTTTGCTGGCGAGCCTGGTCTGGCCAGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACC
GGGCTGATTGCGGCATGGGTCCAGCAGAGTCCGTTAACACTACAAGATGCAGTCACCGGCGTCCTGGTGGGGTTTATCGC
TTTTTATTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG
CGTTAGGTAGTTGGGTGGGGCCGTTGTCGCTACCTAATGTTGCTTTAATCGCCTCATGCTGTGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.693

99.257

0.454
  pilD Vibrio campbellii strain DS40M4

41.697

100

0.42
  pilD Acinetobacter nosocomialis M2

38.491

98.513

0.379
  pilD Neisseria gonorrhoeae MS11

39.3

95.539

0.375
  pilD Acinetobacter baumannii D1279779

38.113

98.513

0.375