Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   N7988_RS21295 Genome accession   NZ_CP106675
Coordinates   4156513..4157556 (-) Length   347 a.a.
NCBI ID   WP_001013186.1    Uniprot ID   -
Organism   Bacillus cereus strain SRCM116293     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4151513..4162556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7988_RS21250 (N7988_21250) - 4151944..4152144 (-) 201 WP_000106083.1 YqzE family protein -
  N7988_RS21255 (N7988_21255) - 4152183..4152680 (-) 498 WP_000836650.1 shikimate kinase -
  N7988_RS21260 (N7988_21260) - 4152796..4153446 (-) 651 WP_000183862.1 2OG-Fe(II) oxygenase -
  N7988_RS21265 (N7988_21265) comGG 4153623..4153994 (-) 372 WP_001231273.1 competence type IV pilus minor pilin ComGG -
  N7988_RS21270 (N7988_21270) comGF 4153991..4154461 (-) 471 WP_000923099.1 competence type IV pilus minor pilin ComGF -
  N7988_RS21275 (N7988_21275) - 4154431..4154733 (-) 303 WP_000829473.1 hypothetical protein -
  N7988_RS21280 (N7988_21280) comGD 4154726..4155181 (-) 456 WP_000813142.1 competence type IV pilus minor pilin ComGD -
  N7988_RS21285 (N7988_21285) comGC 4155178..4155477 (-) 300 WP_001178686.1 competence type IV pilus major pilin ComGC -
  N7988_RS21290 (N7988_21290) comGB 4155489..4156520 (-) 1032 WP_000475192.1 competence type IV pilus assembly protein ComGB -
  N7988_RS21295 (N7988_21295) comGA 4156513..4157556 (-) 1044 WP_001013186.1 competence type IV pilus ATPase ComGA Machinery gene
  N7988_RS21300 (N7988_21300) - 4157762..4158457 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  N7988_RS21305 (N7988_21305) - 4158583..4158825 (+) 243 WP_000440721.1 DUF2626 domain-containing protein -
  N7988_RS21310 (N7988_21310) - 4158932..4160326 (+) 1395 WP_001094334.1 L-cystine transporter -
  N7988_RS21315 (N7988_21315) - 4160406..4160798 (-) 393 WP_098348273.1 hypothetical protein -
  N7988_RS21320 (N7988_21320) - 4160886..4161101 (-) 216 WP_001008326.1 DUF3912 family protein -
  N7988_RS21325 (N7988_21325) - 4161354..4161665 (+) 312 WP_001093237.1 hypothetical protein -
  N7988_RS21330 (N7988_21330) - 4161700..4162188 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39114.45 Da        Isoelectric Point: 8.3536

>NTDB_id=734041 N7988_RS21295 WP_001013186.1 4156513..4157556(-) (comGA) [Bacillus cereus strain SRCM116293]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTSQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=734041 N7988_RS21295 WP_001013186.1 4156513..4157556(-) (comGA) [Bacillus cereus strain SRCM116293]
ATGAATGGGATTGAAATTTTCGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGCCAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGCTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAAGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGGGATGAGGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGATATGAGTTAAAACAAGCACTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCATCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369