Detailed information NTG0733788

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N7918_RS23465
• Genome accession: NZ_CP106658
• Coordinates: 4942449..4943246 (+)
• Length: 265 a.a.
• NCBI ID: WP_032694399.1
• Uniprot ID: A0A2J5PD60
• Organism: Klebsiella michiganensis strain 8BG
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4937449..4948246

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N7918_RS23430 (N7918_23430)	gspH	4937590..4938102 (+)	513	WP_014227977.1	type II secretion system minor pseudopilin GspH	-
N7918_RS23435 (N7918_23435)	gspI	4938099..4938464 (+)	366	WP_032720566.1	type II secretion system minor pseudopilin GspI	-
N7918_RS23440 (N7918_23440)	gspJ	4938461..4939057 (+)	597	WP_262190864.1	type II secretion system minor pseudopilin GspJ	-
N7918_RS23445 (N7918_23445)	gspK	4939054..4940034 (+)	981	WP_014227974.1	type II secretion system minor pseudopilin GspK	-
N7918_RS23450 (N7918_23450)	gspL	4940027..4941220 (+)	1194	WP_032694396.1	type II secretion system protein GspL	-
N7918_RS23455 (N7918_23455)	gspM	4941213..4941698 (+)	486	WP_014837427.1	type II secretion system protein GspM	-
N7918_RS23460 (N7918_23460)	-	4941688..4942446 (+)	759	WP_262190865.1	type II secretion system protein N	-
N7918_RS23465 (N7918_23465)	pilD	4942449..4943246 (+)	798	WP_032694399.1	A24 family peptidase	Machinery gene
N7918_RS23470 (N7918_23470)	hrpB	4943344..4945773 (-)	2430	WP_262190866.1	ATP-dependent helicase HrpB	-
N7918_RS23475 (N7918_23475)	thpR	4945846..4946382 (+)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
N7918_RS23480 (N7918_23480)	sfsA	4946382..4947098 (+)	717	WP_014227967.1	DNA/RNA nuclease SfsA	-
N7918_RS23485 (N7918_23485)	dksA	4947263..4947718 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29150.77 Da
• Isoelectric Point: 8.3410

>NTDB_id=733788 N7918_RS23465 WP_032694399.1 4942449..4943246(+) (pilD) [Klebsiella michiganensis strain 8BG]
MENIALLPEFAAQFPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=733788 N7918_RS23465 WP_032694399.1 4942449..4943246(+) (pilD) [Klebsiella michiganensis strain 8BG]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATTCCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTAAATCTCTGCC
TCCCCGCCTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCAGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGTCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A2J5PD60

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.808	98.491	0.491
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.273	100	0.449
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434