Detailed information NTG0733572

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N5863_RS23260
• Genome accession: NZ_CP104925
• Coordinates: 4935724..4936524 (+)
• Length: 266 a.a.
• NCBI ID: WP_004129059.1
• Uniprot ID: A0AAD0Y356
• Organism: Klebsiella pasteurii strain AHM8C130-1I
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4930724..4941524

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5863_RS23225 (N5863_23220)	gspH	4930865..4931377 (+)	513	WP_284658651.1	type II secretion system minor pseudopilin GspH	-
N5863_RS23230 (N5863_23225)	gspI	4931374..4931739 (+)	366	WP_049115511.1	type II secretion system minor pseudopilin GspI	-
N5863_RS23235 (N5863_23230)	gspJ	4931736..4932332 (+)	597	WP_284658652.1	type II secretion system minor pseudopilin GspJ	-
N5863_RS23240 (N5863_23235)	gspK	4932329..4933309 (+)	981	WP_087828443.1	type II secretion system minor pseudopilin GspK	-
N5863_RS23245 (N5863_23240)	gspL	4933302..4934498 (+)	1197	WP_284658654.1	type II secretion system protein GspL	-
N5863_RS23250 (N5863_23245)	-	4934491..4934976 (+)	486	WP_049123297.1	type II secretion system protein M	-
N5863_RS23255 (N5863_23250)	-	4934966..4935724 (+)	759	WP_112166695.1	type II secretion system protein N	-
N5863_RS23260 (N5863_23255)	pilD	4935724..4936524 (+)	801	WP_004129059.1	A24 family peptidase	Machinery gene
N5863_RS23265 (N5863_23260)	hrpB	4936622..4939051 (-)	2430	WP_123828459.1	ATP-dependent helicase HrpB	-
N5863_RS23270 (N5863_23265)	thpR	4939124..4939660 (+)	537	WP_004129056.1	RNA 2',3'-cyclic phosphodiesterase	-
N5863_RS23275 (N5863_23270)	sfsA	4939660..4940376 (+)	717	WP_004129055.1	DNA/RNA nuclease SfsA	-
N5863_RS23280 (N5863_23275)	dksA	4940541..4940996 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29072.63 Da
• Isoelectric Point: 6.4377

>NTDB_id=733572 N5863_RS23260 WP_004129059.1 4935724..4936524(+) (pilD) [Klebsiella pasteurii strain AHM8C130-1I]
MFENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMAHAEGINLCFPASFCPQCREPIAWRDNIPLLSFLF
LKGRSRCCGEPISPRYPLMELATGALFMLVGYLIPPGIPLLGGLLLLSVLLILATIDAQTQLLPDGLTLPLLWAGLLFNL
SDTFAPLAEAVIGAMAGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQALPQTLLLASASGLVWTLLQRLITR
QSFQQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 801 bp

>NTDB_id=733572 N5863_RS23260 WP_004129059.1 4935724..4936524(+) (pilD) [Klebsiella pasteurii strain AHM8C130-1I]
ATGTTCGAAAACATCGCGTTATTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGG
ACTGGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGCGCACGCAGAGGGAATCAATCTCT
GTTTTCCCGCGTCGTTCTGTCCGCAGTGCCGTGAGCCCATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTCCTGTTT
CTTAAGGGACGTTCGCGCTGCTGCGGGGAACCCATCTCGCCTCGTTATCCTCTGATGGAGCTGGCGACCGGCGCGCTGTT
TATGCTGGTCGGATACCTGATACCTCCGGGCATACCGCTGCTGGGCGGCTTGCTTCTGCTGTCGGTTCTACTGATTCTTG
CTACCATCGACGCGCAAACGCAGCTGCTGCCGGACGGGTTGACGCTACCGCTGCTGTGGGCCGGTCTGCTGTTCAATCTT
TCCGATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGCCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGT
GTTCCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCGGCGCTCGGCGCGTGGCTCGGCT
GGCAGGCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGCCTGGTCTGGACGCTGCTGCAGCGCCTGATAACCCGG
CAGTCGTTCCAGCAGCCTTTGGCCTTCGGGCCTTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATA
A

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (26-111)

• Type IV leader peptidase family (Peptidase_A24) (124-232)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.897	100	0.5
pilD	Neisseria gonorrhoeae MS11	47.431	95.113	0.451
pilD	Vibrio campbellii strain DS40M4	42.909	100	0.444
pilD	Acinetobacter nosocomialis M2	44.015	97.368	0.429
pilD	Acinetobacter baumannii D1279779	43.629	97.368	0.425