Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   N7761_RS06080 Genome accession   NZ_CP104882
Coordinates   1196487..1197038 (-) Length   183 a.a.
NCBI ID   WP_005547469.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain IDHaas10a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1191487..1202038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7761_RS06055 (N7761_06055) - 1191744..1192196 (-) 453 WP_005568016.1 isoprenylcysteine carboxylmethyltransferase family protein -
  N7761_RS06060 (N7761_06060) - 1192249..1192863 (-) 615 WP_014167419.1 YfgM family protein -
  N7761_RS06065 (N7761_06065) hisS 1192874..1194151 (-) 1278 WP_005589329.1 histidine--tRNA ligase -
  N7761_RS06070 (N7761_06070) ispG 1194173..1195276 (-) 1104 WP_005542474.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  N7761_RS06075 (N7761_06075) - 1195288..1196343 (-) 1056 WP_005568019.1 RodZ family helix-turn-helix domain-containing protein -
  N7761_RS06080 (N7761_06080) pilF/pilF2 1196487..1197038 (-) 552 WP_005547469.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  N7761_RS06085 (N7761_06085) - 1197060..1198220 (-) 1161 WP_005586654.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  N7761_RS06090 (N7761_06090) ansB 1198494..1199543 (+) 1050 WP_005571119.1 L-asparaginase 2 -
  N7761_RS06095 (N7761_06095) - 1199724..1201088 (-) 1365 WP_005547462.1 L-serine ammonia-lyase -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20667.46 Da        Isoelectric Point: 8.8869

>NTDB_id=733194 N7761_RS06080 WP_005547469.1 1196487..1197038(-) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain IDHaas10a]
MIKFQQHNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKAFSYAPDYYLVSAAFAYF
YQQQGDIEQARTAYLNAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPKRAAKLPQGIK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=733194 N7761_RS06080 WP_005547469.1 1196487..1197038(-) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain IDHaas10a]
ATGATCAAATTTCAACAGCACAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGCCCGTACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGAAACGGGCGGCAAAATTACCACAAGGGATAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(78-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

57.576

90.164

0.519
  pilF Glaesserella parasuis strain SC1401

47.059

92.896

0.437