Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   N8A75_RS01980 Genome accession   NZ_CP104878
Coordinates   369282..369731 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain 4ZT     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 364282..374731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8A75_RS01940 (N8A75_01940) - 364721..364918 (+) 198 Protein_339 hypothetical protein -
  N8A75_RS01945 (N8A75_01945) - 364955..365275 (+) 321 Protein_340 RDD family protein -
  N8A75_RS01950 (N8A75_01950) yckD 365357..365689 (+) 333 WP_015252849.1 YckD family protein -
  N8A75_RS01955 (N8A75_01955) - 365817..366239 (+) 423 WP_201449129.1 MarR family winged helix-turn-helix transcriptional regulator -
  N8A75_RS01960 (N8A75_01960) - 366227..366568 (+) 342 WP_032724911.1 DUF3147 family protein -
  N8A75_RS01965 (N8A75_01965) - 366587..367315 (+) 729 WP_046160039.1 AAA family ATPase -
  N8A75_RS01970 (N8A75_01970) bglC 367387..368820 (+) 1434 WP_046160040.1 6-phospho-beta-glucosidase -
  N8A75_RS01975 (N8A75_01975) nin/comJ 368857..369255 (-) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  N8A75_RS01980 (N8A75_01980) nucA/comI 369282..369731 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  N8A75_RS01985 (N8A75_01985) tlpC 369899..371620 (-) 1722 WP_046160041.1 methyl-accepting chemotaxis protein TlpC -
  N8A75_RS01990 (N8A75_01990) hxlB 371730..372287 (-) 558 WP_021481820.1 6-phospho-3-hexuloisomerase -
  N8A75_RS01995 (N8A75_01995) hxlA 372293..372925 (-) 633 WP_021481819.1 3-hexulose-6-phosphate synthase -
  N8A75_RS02000 (N8A75_02000) hxlR 373157..373519 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=733090 N8A75_RS01980 WP_014475770.1 369282..369731(-) (nucA/comI) [Bacillus subtilis strain 4ZT]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=733090 N8A75_RS01980 WP_014475770.1 369282..369731(-) (nucA/comI) [Bacillus subtilis strain 4ZT]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987