Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   P5661_RS08095 Genome accession   NZ_CP120600
Coordinates   1506244..1508343 (-) Length   699 a.a.
NCBI ID   WP_038427619.1    Uniprot ID   -
Organism   Bacillus subtilis strain PRO112     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1501244..1513343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5661_RS08065 (P5661_08065) spo0E 1501300..1501557 (+) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -
  P5661_RS08070 (P5661_08070) - 1501643..1502068 (+) 426 WP_277709555.1 hypothetical protein -
  P5661_RS08075 (P5661_08075) kinD 1502102..1503622 (-) 1521 WP_015252216.1 sporulation kinase KinD -
  P5661_RS08080 (P5661_08080) mhqR 1503815..1504252 (+) 438 WP_049139814.1 MarR family transcriptional regulator MhqR -
  P5661_RS08085 (P5661_08085) motB 1504292..1505077 (-) 786 WP_277709556.1 flagellar motor protein MotB -
  P5661_RS08090 (P5661_08090) motA 1505049..1505861 (-) 813 WP_003238984.1 flagellar motor stator protein MotA -
  P5661_RS08095 (P5661_08095) clpE 1506244..1508343 (-) 2100 WP_038427619.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  P5661_RS08100 (P5661_08100) - 1508708..1509751 (+) 1044 WP_072175394.1 hypothetical protein -
  P5661_RS08105 (P5661_08105) queC 1510062..1510721 (+) 660 WP_032725593.1 7-cyano-7-deazaguanine synthase QueC -
  P5661_RS08110 (P5661_08110) queD 1510714..1511163 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  P5661_RS08115 (P5661_08115) queE 1511156..1511887 (+) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  P5661_RS08120 (P5661_08120) queF 1511905..1512402 (+) 498 WP_003218613.1 preQ(1) synthase -
  P5661_RS08125 (P5661_08125) ykvN 1512879..1513238 (-) 360 WP_161621542.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77819.91 Da        Isoelectric Point: 5.1837

>NTDB_id=732962 P5661_RS08095 WP_038427619.1 1506244..1508343(-) (clpE) [Bacillus subtilis strain PRO112]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEKSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEDENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=732962 P5661_RS08095 WP_038427619.1 1506244..1508343(-) (clpE) [Bacillus subtilis strain PRO112]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAAAAGAGCGCAGCAAAACAAAGCGGAAAAAAAGGGTTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCAAAAGCCGGTCTCATTGACCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGAAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTGAAAAACAAAGAGCTATATTTGCTTGATGTTGCATCCCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCATACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGATGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATTGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAGGCGGTAAGACG
AAGCCGCGCCGGTTTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAAGT
GCGCCGCAATCCATACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAGGATGGCCGTCTGACAGACAGCCAAGGCAGAACTGTAAGCTTCAAAGACACAGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTGATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCTGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGGGCGAAATAA

Domains

Predicted by InterProScan.

AAA lid domain (AAA_lid_9)

(253-352)

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.662

100

0.602
  clpC Lactococcus lactis subsp. cremoris KW2

56.284

100

0.589
  clpE Streptococcus pneumoniae TIGR4

54.785

100

0.565
  clpE Streptococcus pneumoniae Rx1

54.785

100

0.565
  clpE Streptococcus pneumoniae D39

54.785

100

0.565
  clpE Streptococcus pneumoniae R6

54.785

100

0.565
  clpC Bacillus subtilis subsp. subtilis str. 168

54.016

90.844

0.491
  clpC Streptococcus pneumoniae Rx1

45.884

93.848

0.431
  clpC Streptococcus pneumoniae D39

45.884

93.848

0.431
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.687

89.7

0.428
  clpC Streptococcus thermophilus LMD-9

45.427

93.848

0.426
  clpC Streptococcus thermophilus LMG 18311

44.97

93.848

0.422
  clpC Streptococcus pneumoniae TIGR4

47.011

88.555

0.416
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

41.749

86.695

0.362