Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N7D90_RS22700 Genome accession   NZ_CP104861
Coordinates   4955009..4956343 (-) Length   444 a.a.
NCBI ID   WP_315968130.1    Uniprot ID   -
Organism   Pseudomonas fragi strain D12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4950009..4961343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7D90_RS22685 (N7D90_22685) - 4951648..4953213 (-) 1566 WP_261740071.1 SPOR domain-containing protein -
  N7D90_RS22690 (N7D90_22690) aroB 4953224..4954324 (-) 1101 WP_261740072.1 3-dehydroquinate synthase -
  N7D90_RS22695 (N7D90_22695) aroK 4954486..4955004 (-) 519 WP_261740073.1 shikimate kinase AroK -
  N7D90_RS22700 (N7D90_22700) pilQ 4955009..4956343 (-) 1335 WP_315968130.1 type IV pilus secretin PilQ family protein Machinery gene
  N7D90_RS22705 (N7D90_22705) - 4956357..4956884 (-) 528 WP_261740074.1 pilus assembly protein PilP -
  N7D90_RS22710 (N7D90_22710) - 4956881..4957504 (-) 624 WP_261740075.1 type 4a pilus biogenesis protein PilO -
  N7D90_RS22715 (N7D90_22715) - 4957501..4958067 (-) 567 WP_261740076.1 PilN domain-containing protein -
  N7D90_RS22720 (N7D90_22720) - 4958067..4959029 (-) 963 WP_261740077.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 48053.06 Da        Isoelectric Point: 4.9703

>NTDB_id=732823 N7D90_RS22700 WP_315968130.1 4955009..4956343(-) (pilQ) [Pseudomonas fragi strain D12]
MKRILPVCVLALWSALNSQSTLALVQAAPSSVATNPPYVGEKMSLNFQNVEVRTVLQLLADFTGFNLVVSDAVQGSITLN
LQDVPWDQALDLVLESKDLGKRLEGNVLRIAPAEHLAARAQHDFAPLRRELVQVNYAKAVDLVRLFQSVTATQPGINGQG
TITVDERTNNIIAFESGERLDELRRIVAQLDVPVRQVMIEARIVEAGVDFEKSLGVSWGGTLRRGGNWNAGGITRSAASG
GALDNMSAPFVDLGVSGNTSGLGIAFITNNVLLDLELTAMEKTGNGEIISQPKVVTSDKETAKILKGTEIPYQETSASGA
TSVSFKEASLSLEVTPQITPDNSVIMDVIVTKDEPDYMNRIQDVPPIKKNEVRAKVLVKDGETIVIGGVFSNTQSKVIDK
VPFLGDLPYVGQLFRKDVVLEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=732823 N7D90_RS22700 WP_315968130.1 4955009..4956343(-) (pilQ) [Pseudomonas fragi strain D12]
ATGAAAAGGATTTTACCGGTCTGCGTACTGGCGCTATGGAGTGCGCTGAATTCGCAGTCAACACTGGCGCTGGTACAGGC
TGCCCCCTCCTCAGTCGCCACGAACCCGCCGTATGTGGGCGAGAAAATGTCCCTGAACTTTCAGAATGTGGAAGTACGCA
CAGTGCTTCAGCTACTGGCAGATTTTACCGGGTTTAACCTGGTGGTCAGCGACGCCGTGCAAGGCAGCATTACGCTCAAC
TTGCAGGATGTACCGTGGGATCAGGCGCTGGATCTGGTACTTGAAAGCAAGGATTTGGGTAAACGCCTTGAGGGCAATGT
GTTGCGCATAGCGCCTGCTGAGCACCTCGCGGCCCGCGCGCAGCATGACTTTGCGCCTTTGCGTCGAGAGCTGGTGCAGG
TCAATTACGCCAAGGCGGTAGATCTCGTCAGGTTGTTCCAGTCGGTGACCGCCACTCAGCCCGGTATCAACGGGCAGGGC
ACGATCACGGTGGATGAGCGCACCAATAACATCATTGCCTTTGAGTCGGGCGAGCGGCTGGATGAGTTACGGCGCATCGT
GGCCCAACTGGATGTGCCAGTACGTCAGGTGATGATCGAGGCCAGGATTGTTGAGGCGGGCGTGGACTTCGAGAAAAGCC
TGGGAGTGAGTTGGGGCGGGACCTTGCGTAGAGGCGGTAACTGGAATGCTGGCGGCATTACCCGGTCTGCTGCGTCAGGG
GGGGCGCTTGACAACATGAGTGCGCCCTTTGTGGATCTGGGGGTTTCGGGCAATACATCGGGGTTGGGCATTGCCTTTAT
CACCAACAACGTACTGTTGGACCTGGAGCTGACGGCTATGGAAAAAACCGGCAATGGCGAAATCATCTCTCAGCCCAAAG
TGGTTACTTCAGACAAAGAGACCGCCAAAATCCTTAAAGGCACCGAAATCCCCTATCAGGAGACCAGCGCAAGCGGGGCC
ACCTCGGTGTCGTTCAAGGAAGCGTCGCTGTCGCTGGAGGTGACGCCGCAAATCACCCCGGACAATAGCGTCATCATGGA
TGTCATCGTGACCAAGGATGAGCCCGATTACATGAACAGGATCCAGGATGTGCCACCAATCAAGAAGAACGAAGTCAGGG
CCAAGGTGCTGGTCAAGGACGGTGAAACCATCGTGATTGGGGGAGTGTTCTCAAATACTCAAAGCAAAGTAATAGATAAA
GTGCCATTTTTAGGCGATCTGCCGTATGTTGGCCAACTTTTCCGTAAAGACGTTGTCCTGGAAAAAAAATCCGAACTGCT
GGTGTTCCTGACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(65-112)

Bacterial type II and III secretion system protein (Secretin)

(279-435)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

64.76

98.423

0.637
  comQ Acinetobacter baylyi ADP1

44.053

100

0.45
  pilQ Acinetobacter baumannii strain A118

41.423

100

0.446
  pilQ Acinetobacter baumannii D1279779

41.423

100

0.446
  pilQ Legionella pneumophila strain Lp02

43.571

94.595

0.412
  pilQ Legionella pneumophila strain ERS1305867

43.333

94.595

0.41
  pilQ Vibrio campbellii strain DS40M4

40.662

95.27

0.387
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.205

93.468

0.385
  pilQ Vibrio cholerae strain A1552

41.205

93.468

0.385