Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   NAL19_RS14810 Genome accession   NZ_CP104733
Coordinates   3317246..3318091 (-) Length   281 a.a.
NCBI ID   WP_263057740.1    Uniprot ID   -
Organism   Pectobacterium sp. F1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3312246..3323091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAL19_RS14765 (NAL19_3198) dbpA 3312513..3313895 (-) 1383 WP_263057738.1 ATP-dependent RNA helicase DbpA -
  NAL19_RS14770 (NAL19_3199) - 3314014..3314388 (-) 375 WP_263057739.1 substrate-binding domain-containing protein -
  NAL19_RS14775 (NAL19_3200) - 3314390..3314524 (-) 135 WP_411561400.1 hypothetical protein -
  NAL19_RS14780 (NAL19_3201) - 3314524..3314775 (-) 252 Protein_2909 iron chelate uptake ABC transporter family permease subunit -
  NAL19_RS14785 (NAL19_3202) - 3314772..3315056 (-) 285 Protein_2910 hypothetical protein -
  NAL19_RS14790 - 3315046..3316071 (+) 1026 Protein_2911 LLM class flavin-dependent oxidoreductase -
  NAL19_RS14795 ypdK 3316431..3316505 (+) 75 WP_099606229.1 membrane protein YpdK -
  NAL19_RS14800 - 3316725..3316931 (-) 207 WP_225088193.1 YciI family protein -
  NAL19_RS14805 - 3317035..3317142 (-) 108 Protein_2914 hypothetical protein -
  NAL19_RS14810 (NAL19_3206) pilD 3317246..3318091 (-) 846 WP_263057740.1 prepilin peptidase Machinery gene
  NAL19_RS14815 (NAL19_3207) - 3318199..3318885 (-) 687 WP_263057741.1 type II secretion system protein N -
  NAL19_RS14820 (NAL19_3208) - 3318937..3319431 (-) 495 WP_263057742.1 type II secretion system protein M -
  NAL19_RS14825 (NAL19_3209) gspL 3319428..3320705 (-) 1278 WP_263057743.1 type II secretion system protein GspL -
  NAL19_RS14830 (NAL19_3210) gspK 3320702..3321688 (-) 987 WP_225085310.1 type II secretion system minor pseudopilin GspK -
  NAL19_RS14835 (NAL19_3211) gspJ 3321685..3322335 (-) 651 WP_225085311.1 type II secretion system minor pseudopilin GspJ -
  NAL19_RS14840 (NAL19_3212) gspI 3322335..3322700 (-) 366 WP_180741890.1 type II secretion system minor pseudopilin GspI -

Sequence

Protein


Download         Length: 281 a.a.        Molecular weight: 31322.33 Da        Isoelectric Point: 7.9960

>NTDB_id=731659 NAL19_RS14810 WP_263057740.1 3317246..3318091(-) (pilD) [Pectobacterium sp. F1-1]
MDDLREFAQVFPAWWYGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELQTGVTDAGQATRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCYQPVSVQYPLVEAITMLAFLAAGLLWLPGMALCAALILLSFLVVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLRDAVVGAMAGYLSLWLLYWVFKYATGKDALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLIVTLIWRGLRKEDTAKPLAFGPWLAIGGVFSVMMNGFNL

Nucleotide


Download         Length: 846 bp        

>NTDB_id=731659 NAL19_RS14810 WP_263057740.1 3317246..3318091(-) (pilD) [Pectobacterium sp. F1-1]
GTGGATGATTTAAGGGAATTCGCACAGGTGTTTCCAGCATGGTGGTATGGTGCGCTGGGCGTATTGGGATTGATTGTTGG
CAGCTTTCTGAACGTAGTGATTTATCGCTTGCCGATTATGTTGGAACGGCGCTGGCGGCAAGACATCGAGCTGCAAACCG
GCGTAACGGATGCCGGGCAGGCTACGCGCTATAACCTGTGGTGGCCGCCTTCGTCCTGCCCGCACTGCCAGCAGGCGATT
GCGGTGAAGGATAACATCCCGTTGTTCAGTTGGCTGTGGCTACGCGGTCGCTCCCGCTGCTGTTATCAACCGGTGTCTGT
ACAGTATCCGCTGGTGGAAGCCATCACCATGCTGGCTTTTCTGGCTGCGGGGTTGCTGTGGCTGCCCGGTATGGCGCTGT
GTGCGGCGTTAATCCTGCTGTCCTTTCTGGTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTCTTGTGGATAGGGCTATTGTTTAACCTGTCGGAGACATTTGTTCCATTACGTGATGCCGTGGTCGGTGC
GATGGCGGGCTATTTGTCCCTGTGGCTACTTTACTGGGTATTTAAATATGCCACTGGCAAAGACGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCACTGGGAGCCTGGCTGGGCTGGCAGGCATTACCGAATCTGGTTTTGGTGGCGGCGCTGAGT
GGTCTGATCGTGACGCTTATTTGGCGTGGATTGCGTAAAGAAGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATAGGTGGCGTGTTCAGCGTGATGATGAACGGGTTCAATCTGTAG

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

50.37

96.085

0.484
  pilD Neisseria gonorrhoeae MS11

48.047

91.103

0.438
  pilD Vibrio campbellii strain DS40M4

45.725

95.73

0.438
  pilD Acinetobacter baumannii D1279779

43.629

92.171

0.402
  pilD Acinetobacter nosocomialis M2

43.629

92.171

0.402