Detailed information NTG0731286

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N5936_RS24015
• Genome accession: NZ_CP104659
• Coordinates: 4954547..4955347 (-)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain 2020CK-00096
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4949547..4960347

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5936_RS23995 (N5936_23995)	dksA	4950127..4950582 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
N5936_RS24000 (N5936_24000)	sfsA	4950745..4951461 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
N5936_RS24005 (N5936_24005)	thpR	4951461..4951997 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
N5936_RS24010 (N5936_24010)	hrpB	4952070..4954499 (+)	2430	WP_032434212.1	ATP-dependent helicase HrpB	-
N5936_RS24015 (N5936_24015)	pilD	4954547..4955347 (-)	801	WP_004213441.1	prepilin peptidase	Machinery gene
N5936_RS24020 (N5936_24020)	-	4955344..4956090 (-)	747	WP_032434210.1	type II secretion system protein N	-
N5936_RS24025 (N5936_24025)	-	4956080..4956565 (-)	486	WP_004145896.1	type II secretion system protein M	-
N5936_RS24030 (N5936_24030)	gspL	4956558..4957751 (-)	1194	WP_032434208.1	type II secretion system protein GspL	-
N5936_RS24035 (N5936_24035)	gspK	4957738..4958724 (-)	987	WP_077254116.1	type II secretion system minor pseudopilin GspK	-
N5936_RS24040 (N5936_24040)	gspJ	4958721..4959317 (-)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
N5936_RS24045 (N5936_24045)	gspI	4959314..4959679 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
N5936_RS24050 (N5936_24050)	gspH	4959676..4960191 (-)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=731286 N5936_RS24015 WP_004213441.1 4954547..4955347(-) (pilD) [Klebsiella pneumoniae strain 2020CK-00096]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=731286 N5936_RS24015 WP_004213441.1 4954547..4955347(-) (pilD) [Klebsiella pneumoniae strain 2020CK-00096]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425