Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N4G50_RS03945 Genome accession   NZ_CP104479
Coordinates   816678..817658 (+) Length   326 a.a.
NCBI ID   WP_024156681.1    Uniprot ID   A0A745GFS1
Organism   Salmonella enterica strain SalSpp_sample_09_No.5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 811678..822658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4G50_RS03915 (N4G50_03915) - 812256..813263 (+) 1008 WP_024156684.1 DUF1202 domain-containing protein -
  N4G50_RS03920 (N4G50_03920) hemW 813354..814490 (-) 1137 WP_024156683.1 radical SAM family heme chaperone HemW -
  N4G50_RS03925 (N4G50_03925) - 814483..815076 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  N4G50_RS03930 (N4G50_03930) yggU 815084..815374 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  N4G50_RS03935 (N4G50_03935) - 815371..815937 (-) 567 WP_001094848.1 YggT family protein -
  N4G50_RS03940 (N4G50_03940) - 815956..816660 (-) 705 WP_024156682.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4G50_RS03945 (N4G50_03945) pilT 816678..817658 (+) 981 WP_024156681.1 type IV pilus twitching motility protein PilT Machinery gene
  N4G50_RS03950 (N4G50_03950) - 817791..818477 (+) 687 WP_000098324.1 IclR family transcriptional regulator -
  N4G50_RS03955 (N4G50_03955) ruvX 818524..818940 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  N4G50_RS03960 (N4G50_03960) - 818940..819503 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  N4G50_RS03965 (N4G50_03965) gshB 819719..820666 (-) 948 WP_000593248.1 glutathione synthase -
  N4G50_RS03970 (N4G50_03970) rsmE 820686..821417 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N4G50_RS03975 (N4G50_03975) endA 821494..822201 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36110.31 Da        Isoelectric Point: 7.5355

>NTDB_id=729906 N4G50_RS03945 WP_024156681.1 816678..817658(+) (pilT) [Salmonella enterica strain SalSpp_sample_09_No.5]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRAHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=729906 N4G50_RS03945 WP_024156681.1 816678..817658(+) (pilT) [Salmonella enterica strain SalSpp_sample_09_No.5]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACATCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAGCGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGTGCTACTGGCTGCGGAAAGT
CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A745GFS1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

45.455

97.853

0.445
  pilT Pseudomonas aeruginosa PAK

45.886

96.933

0.445
  pilT Pseudomonas stutzeri DSM 10701

45.886

96.933

0.445
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.952

100

0.365