Detailed information NTG0729743

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N2J37_RS23250
• Genome accession: NZ_CP104450
• Coordinates: 4770852..4771649 (+)
• Length: 265 a.a.
• NCBI ID: WP_260990335.1
• Uniprot ID: A0A9Q9JF09
• Organism: Raoultella ornithinolytica strain MQB_Silv_108
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4765852..4776649

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N2J37_RS23215 (N2J37_23215)	gspH	4765972..4766490 (+)	519	WP_015585120.1	type II secretion system minor pseudopilin GspH	-
N2J37_RS23220 (N2J37_23220)	gspI	4766487..4766852 (+)	366	WP_260990333.1	type II secretion system minor pseudopilin GspI	-
N2J37_RS23225 (N2J37_23225)	gspJ	4766864..4767448 (+)	585	WP_088883435.1	type II secretion system minor pseudopilin GspJ	-
N2J37_RS23230 (N2J37_23230)	gspK	4767445..4768431 (+)	987	WP_054829280.1	type II secretion system minor pseudopilin GspK	-
N2J37_RS23235 (N2J37_23235)	gspL	4768418..4769620 (+)	1203	WP_260990334.1	type II secretion system protein GspL	-
N2J37_RS23240 (N2J37_23240)	gspM	4769613..4770107 (+)	495	WP_123629630.1	type II secretion system protein GspM	-
N2J37_RS23245 (N2J37_23245)	-	4770097..4770837 (+)	741	WP_032716580.1	type II secretion system protein N	-
N2J37_RS23250 (N2J37_23250)	pilD	4770852..4771649 (+)	798	WP_260990335.1	A24 family peptidase	Machinery gene
N2J37_RS23255 (N2J37_23255)	hrpB	4771670..4774099 (-)	2430	WP_064359573.1	ATP-dependent helicase HrpB	-
N2J37_RS23260 (N2J37_23260)	thpR	4774172..4774708 (+)	537	WP_004858022.1	RNA 2',3'-cyclic phosphodiesterase	-
N2J37_RS23265 (N2J37_23265)	sfsA	4774708..4775424 (+)	717	WP_004858020.1	DNA/RNA nuclease SfsA	-
N2J37_RS23270 (N2J37_23270)	dksA	4775589..4776044 (+)	456	WP_004858018.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29345.98 Da
• Isoelectric Point: 8.4762

>NTDB_id=729743 N2J37_RS23250 WP_260990335.1 4770852..4771649(+) (pilD) [Raoultella ornithinolytica strain MQB_Silv_108]
MTLLSAFSLQFPLLWGGLLFIFGLTFGSFFNVVIYRLPLMMQQEESARFNLCVPASFCPQCQRPIIWRDNIPLLSYLSLK
GRARCCQAPISQRYPLTELASGLLFVLTGYLLTPGLPLLGGLILLSTLLVLAIIDGQTQLLPDRLTLPLLWAGLLFNLNG
TFVPLSEAVIGAMTGYLSLWTVYWLFRLLTGKEALGYGDFKLLAALGAWSGWQILPQTLLCASASGLIWTLLQRRITLQS
LDQPLAFGPWLALAGSSLFLWTQTL

Nucleotide

• Length: 798 bp

>NTDB_id=729743 N2J37_RS23250 WP_260990335.1 4770852..4771649(+) (pilD) [Raoultella ornithinolytica strain MQB_Silv_108]
ATGACGCTACTGAGCGCATTTTCGTTGCAGTTCCCCCTGTTATGGGGCGGATTGTTGTTTATTTTCGGCCTGACGTTTGG
CAGCTTTTTTAACGTGGTTATCTACCGTTTGCCGCTGATGATGCAGCAGGAAGAGTCCGCACGATTCAACCTGTGCGTTC
CCGCCTCCTTCTGCCCCCAGTGCCAGCGGCCCATCATCTGGCGGGATAATATTCCGCTGCTCAGCTATCTCTCACTCAAA
GGGCGCGCCCGCTGCTGCCAGGCGCCCATTTCTCAGCGCTATCCGCTGACGGAACTGGCCAGCGGACTGCTCTTTGTGTT
GACCGGCTATCTGCTGACGCCGGGATTACCGCTGCTGGGGGGCCTGATCCTGCTCTCCACGCTGCTGGTACTGGCGATCA
TCGACGGCCAGACTCAGCTCCTGCCGGATCGGCTGACTCTGCCGCTGCTGTGGGCCGGTCTGCTGTTTAATCTCAACGGC
ACGTTTGTCCCGCTCAGCGAAGCGGTGATCGGCGCCATGACGGGCTATTTATCACTGTGGACGGTTTACTGGCTGTTTCG
CCTGCTGACCGGCAAAGAGGCGCTAGGATACGGCGATTTTAAGCTGCTGGCGGCACTCGGCGCATGGTCCGGCTGGCAGA
TCCTGCCGCAGACGCTGCTGTGCGCCTCGGCCAGCGGCCTGATCTGGACCTTGCTGCAACGGCGGATCACGCTCCAGTCG
CTGGATCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCAGCAGCCTGTTTCTGTGGACTCAGACGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (122-230)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.529	100	0.498
pilD	Vibrio campbellii strain DS40M4	46.183	98.868	0.457
pilD	Neisseria gonorrhoeae MS11	46.275	96.226	0.445
pilD	Acinetobacter baumannii D1279779	44.574	97.358	0.434
pilD	Acinetobacter nosocomialis M2	44.186	97.358	0.43