Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   N4562_RS08620 Genome accession   NZ_CP104396
Coordinates   1712983..1714311 (-) Length   442 a.a.
NCBI ID   WP_260903434.1    Uniprot ID   A0A9Q9J6H4
Organism   Ligilactobacillus agilis strain AM_LB6     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1707983..1719311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4562_RS08605 (N4562_08605) secA 1709033..1711396 (-) 2364 WP_089144729.1 preprotein translocase subunit SecA -
  N4562_RS08610 (N4562_08610) hpf 1711571..1712125 (-) 555 WP_050610924.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  N4562_RS08615 (N4562_08615) - 1712286..1712990 (-) 705 WP_260903431.1 ComF family protein -
  N4562_RS08620 (N4562_08620) comFA 1712983..1714311 (-) 1329 WP_260903434.1 DEAD/DEAH box helicase Machinery gene
  N4562_RS08625 (N4562_08625) - 1714370..1715017 (+) 648 WP_170091278.1 YigZ family protein -
  N4562_RS08630 (N4562_08630) - 1715056..1716174 (-) 1119 WP_089144732.1 glycosyltransferase family 4 protein -
  N4562_RS08635 (N4562_08635) - 1716372..1718207 (-) 1836 WP_089144733.1 APC family permease -

Sequence

Protein


Download         Length: 442 a.a.        Molecular weight: 49645.03 Da        Isoelectric Point: 10.1190

>NTDB_id=729533 N4562_RS08620 WP_260903434.1 1712983..1714311(-) (comFA) [Ligilactobacillus agilis strain AM_LB6]
MDASILYGRQIVLGEKGLEGLNNLRVKPALVVGKRGVICQRCGQTTSLKLARLPTGKYYCPNCLLLGRLTSADRLYNLPE
PHLFEYQKNSLTWQGQLSKYQEKCSAQLQTVVKHGGKHLLWAVTGAGKTEMLFETINWALQTGKRVGLASPRVDVCNELF
PRLQAAFAKTPMILLHGHAKEPYRYTQLVVATTHQLLRFYQAFDLLIIDEVDAFPFVDDQGLHYAANNALIPGGTLVYLT
ATPSRELLAAVKKKELSVSYLPLRFHGYLLPTINLVAVAKLRSQLDEGILNSKIKKILLRWQEEGYQFLVFVPRIALLKP
VYQAIANLLKPELKGQTVYANDGQRIEKVAKMRAQEYRYLVTTTILERGVTFPGINIMVLAADDSNFSTAALVQIAGRAG
RNKARPTGDVYFFCQDYTRTVKAACKQIKFLNRKGKKLKQDE

Nucleotide


Download         Length: 1329 bp        

>NTDB_id=729533 N4562_RS08620 WP_260903434.1 1712983..1714311(-) (comFA) [Ligilactobacillus agilis strain AM_LB6]
ATGGATGCAAGCATTTTGTACGGTCGCCAAATAGTTTTGGGTGAAAAAGGCTTGGAGGGCTTAAATAATCTCCGGGTTAA
ACCTGCTTTGGTGGTAGGTAAGCGAGGAGTGATTTGTCAAAGGTGCGGGCAGACTACTAGTTTAAAGTTAGCCCGGCTTC
CAACTGGAAAGTACTATTGTCCTAATTGCCTACTGTTAGGACGGCTGACTAGTGCTGACCGGCTTTATAATTTACCAGAG
CCCCACCTATTTGAATACCAAAAAAATAGTTTAACTTGGCAGGGACAATTATCTAAGTACCAGGAGAAGTGCTCGGCTCA
ACTACAAACGGTTGTTAAACACGGGGGCAAACATTTACTGTGGGCTGTAACGGGGGCGGGAAAGACGGAGATGCTTTTTG
AAACCATTAACTGGGCCCTCCAAACTGGAAAGCGGGTGGGACTTGCTTCACCGAGGGTCGATGTTTGTAACGAATTATTT
CCCAGGTTACAAGCTGCATTTGCAAAAACCCCCATGATTTTACTTCACGGCCACGCTAAGGAGCCATACCGTTATACCCA
GCTAGTAGTTGCAACCACCCACCAGCTATTGCGTTTTTACCAGGCATTTGACCTGTTGATAATTGATGAAGTCGATGCTT
TTCCCTTTGTAGATGATCAAGGACTTCATTATGCCGCCAATAACGCGCTAATCCCAGGCGGTACGCTAGTCTACCTAACT
GCAACTCCCAGTAGGGAGCTGTTAGCGGCTGTTAAGAAAAAAGAACTCTCGGTCAGTTACCTCCCACTTCGATTTCATGG
CTACCTACTGCCAACAATTAACTTAGTCGCAGTTGCTAAATTACGCAGTCAGCTTGACGAGGGAATTTTGAATTCAAAAA
TTAAAAAAATTCTACTAAGGTGGCAGGAAGAAGGATATCAATTTTTAGTTTTTGTTCCAAGAATTGCCCTCTTAAAGCCG
GTCTACCAAGCTATTGCTAACTTGTTAAAGCCAGAATTAAAGGGGCAGACGGTCTACGCTAACGATGGTCAACGAATTGA
GAAGGTAGCTAAAATGCGGGCGCAGGAATACCGGTACTTAGTTACGACTACTATTTTGGAGCGGGGGGTAACATTTCCTG
GGATTAATATTATGGTTTTAGCTGCAGACGATAGCAACTTTTCGACGGCTGCTTTGGTCCAGATTGCAGGACGAGCGGGG
AGAAATAAGGCGCGACCTACCGGGGATGTTTATTTCTTTTGTCAAGATTATACCCGGACGGTAAAAGCCGCCTGCAAGCA
AATTAAGTTTCTCAATCGTAAGGGAAAGAAGTTGAAGCAAGATGAATAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(294-402)

Type III restriction enzyme, res subunit (ResIII)

(96-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Latilactobacillus sakei subsp. sakei 23K

48.409

99.548

0.482
  comFA/cflA Streptococcus pneumoniae TIGR4

41.606

92.986

0.387
  comFA/cflA Streptococcus pneumoniae D39

41.363

92.986

0.385
  comFA/cflA Streptococcus pneumoniae Rx1

41.363

92.986

0.385
  comFA/cflA Streptococcus pneumoniae R6

41.363

92.986

0.385
  comFA/cflA Streptococcus mitis NCTC 12261

41.667

92.308

0.385
  comFA Lactococcus lactis subsp. cremoris KW2

38.71

98.19

0.38
  comFA/cflA Streptococcus mitis SK321

40.931

92.308

0.378