Detailed information NTG0729415

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: N4Q31_RS05305
• Genome accession: NZ_CP104391
• Coordinates: 1063638..1067342 (+)
• Length: 1234 a.a.
• NCBI ID: WP_106025296.1
• Uniprot ID: -
• Organism: Bacillus safensis strain GX-H6
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1058638..1072342

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4Q31_RS05290 (N4Q31_05290)	-	1058944..1059354 (+)	411	WP_039177642.1	DoxX family protein	-
N4Q31_RS05295 (N4Q31_05295)	-	1059552..1059998 (-)	447	WP_039177646.1	ferritin-like domain-containing protein	-
N4Q31_RS05300 (N4Q31_05300)	addB	1060151..1063660 (+)	3510	WP_106025297.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
N4Q31_RS05305 (N4Q31_05305)	addA	1063638..1067342 (+)	3705	WP_106025296.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
N4Q31_RS05310 (N4Q31_05310)	sbcD	1067368..1068543 (+)	1176	WP_106025295.1	exonuclease subunit SbcD	-
N4Q31_RS05315 (N4Q31_05315)	sbcC	1068540..1071935 (+)	3396	WP_106025294.1	exonuclease subunit SbcC	-
N4Q31_RS05320 (N4Q31_05320)	-	1071955..1072254 (+)	300	WP_039177660.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 142326.36 Da
• Isoelectric Point: 4.9929

>NTDB_id=729415 N4Q31_RS05305 WP_106025296.1 1063638..1067342(+) (addA) [Bacillus safensis strain GX-H6]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQNDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATEARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFEKAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHAADGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFTPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESEDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMAMIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYERSKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPIVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGDVVLSHPSAFTISFTQASDL
LKEDMSLEKEQQDEVVQALMEGLPVEEYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRKAR
ATLYDRPAFMKKKALTAAEQGTAMHTVMQHIALPSDEPYDDSRIQQLLDSLQQRDLLTNEQVQSIHQEGIAAFFSTSIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRFTGGLEAAVPALKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNIEISL

Nucleotide

• Length: 3705 bp

>NTDB_id=729415 N4Q31_RS05305 WP_106025296.1 1063638..1067342(+) (addA) [Bacillus safensis strain GX-H6]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAATGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCAGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCAGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCGAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
TACCTTGCACTCCTTTTGTTTGCAGGTTCTGCGCACCTTTTATTATGAAATTGACCTTGATCCAGGCTTCCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCG
TTCTTTGAACTGGTTGATCGCTATACTTCTGACCGCCATGATTTAGACTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTTCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGATCTTTCTCTCGTTTTTCGAAGCTGTCTCGAACTACTCGAACGAGCT
CTCGCCCTATCAAAAGAACCGGGCGGCCCGGCACCGAGAGCAGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCAGGCACCAAAATGATTTTGAAAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGAAGCACGCAATCAAGCAAAAAAACAATTAGAAAAACTTAAA
GATGAATACTTTATGCGCAGTCCAGCCCAGCATTTGAAAAGCTTAGCTGAAATGAAGCCGGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAAAGCGAAGCAAGAAAAATCAATCGTGGACTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGATGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAAGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAAGGGGA
ACATGCCGCAGACGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGCTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACAATTTACACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCGAAGATACAACGACAGAAATGCTTCTTGTCCATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGAGAAGAACTAGAGACCGTACAACTAGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTGCTTCGTTCTATGCCATGGGCCCCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCCTCCGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTCTGTTAAAAGTCATTGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCACTTGTCCATTTAGACGAAAATGAAATGGCAATGATTCGCACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGGTTTTTCAGATTGCTGCGTAAATGGCGTGATTTCTCGATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAATCTCCGCGCTCTTTATGA
ACGATCAAAGCAGTATGAAAAAGCTGCCTTTAGAGGACTGTTCCGTTTTCTTCGTTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAAGATGTTGTTCGCATGATGACAATTCACAGCAGTAAAGGG
TTAGAATTTCCAATCGTCTTTACCGCAGGACTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCCTATTTACTTGA
TAAAGAGCTAGGTTTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTAGCGATGA
AAAAAAAGATGAGGAAAGAGCTGTTATCTGAAGAGCTGAGAGTTCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAAGATTGGCTGCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAACAGGTGATGTTGTTCTTTCGCATCCATCAGCATTCACAATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGGAGCAGCAAGACGAGGTGGTACAAGCTTTGATGGAGGGGCTGCCTGTTGAAGA
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCATATCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAGAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAAAGCTCGT
GCCACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAGCACTCACTGCCGCAGAGCAAGGCACCGCAATGCACACAGT
CATGCAGCATATTGCGCTTCCTTCTGACGAGCCCTACGATGATTCACGTATACAGCAGCTCCTCGATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAGCAAGTCCAGTCAATTCATCAAGAAGGAATTGCAGCTTTCTTCTCTACTTCTATTGGTCAA
AAGCTGCGAAAAGCGGATTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGACGGAAAGCTATATTTACTAG
ATTATAAAACCGATAGAGTGCAAGGCAGATTTACAGGCGGCCTTGAAGCAGCTGTGCCTGCATTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGGAATATAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1226)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-462)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	65.698	100	0.66